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Literature summary for 3.3.2.14 extracted from

  • Karthikeyan, S.; Kurt, Z.; Pandey, G.; Spain, J.C.
    Immobilized biocatalyst for detection and destruction of the insensitive explosive, 2,4-dinitroanisole (DNAN) (2016), Environ. Sci. Technol., 50, 11193-11199 .
    View publication on PubMed
Search for more literature for 3.3.2.14

Application

Application Comment Organism
environmental protection the immobilized enzyme can be used as biocatalyst for detection and destruction of the insensitive explosive, 2,4-dinitroanisole (DNAN), with a wide spectrum of applications ranging from national security and demilitarization to environmental monitoring and restoration Nocardioides sp. JS1661

Protein Variants

Protein Variants Comment Organism
additional information enzyme immobilization by biogenic silica encapsulation which is used to stabilize the enzyme and to enable it to be packed into a model microcolumn for application as a biosensor or as a bioreactor for continuous destruction of 2,4-dinitroanisole (DNAN). The immobilized enzyme is stable and not inhibited by other insensitive munitions constituents. The highly specific, convenient and robust biocatalyst DNAN demethylase can mineralize DNAN. An alternative method for DNAN detection involved coating the encapsulated enzyme on cellulose filter paper. Methods, detailed overview Nocardioides sp. JS1661

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dinitroanisole + H2O Nocardioides sp. JS1661
-
 methanol + 2,4-dinitrophenol
-
 ?

Organism

Organism UniProt Comment Textmining
Nocardioides sp. JS1661 A0A096ZEC9 AND A0A096ZED0 subunit alpha and subunit beta, encoded by genes dnhA and dnhB
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain JS1661 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration Nocardioides sp. JS1661

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitroanisole + H2O
-
 Nocardioides sp. JS1661 methanol + 2,4-dinitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
DNAN O-demethylase
-
 Nocardioides sp. JS1661

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Nocardioides sp. JS1661

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Nocardioides sp. JS1661

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme requires no cofactors Nocardioides sp. JS1661