BRENDA - Enzyme Database
show all sequences of 3.3.2.13

In vitro production and purification of isochorismate using a two-enzyme cascade

Hubrich, F.; Mueller, M.; Andexer, J.N.; J. Biotechnol. 191, 93-98 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
chorismate + H2O
Streptomyces hygroscopicus
strict preference for chorismate
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces hygroscopicus
-
subsp. ascomyceticus
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA affinity column chromatography
Streptomyces hygroscopicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
chorismate + H2O
strict preference for chorismate
732211
Streptomyces hygroscopicus
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
-
-
-
?
additional information
isochorismate is not accepted as a substrate
732211
Streptomyces hygroscopicus
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
fkbO
-
Streptomyces hygroscopicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
chorismate + H2O
Streptomyces hygroscopicus
strict preference for chorismate
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA affinity column chromatography
Streptomyces hygroscopicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
chorismate + H2O
strict preference for chorismate
732211
Streptomyces hygroscopicus
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
-
-
-
?
additional information
isochorismate is not accepted as a substrate
732211
Streptomyces hygroscopicus
?
-
-
-
-
Other publictions for EC 3.3.2.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753274
Zhang
How chorismatases regulate di ...
Streptomyces hygroscopicus
Chemistry
25
1326-1336
2019
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2
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-
1
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1
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1
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2
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1
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2
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1
1
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-
755348
Dong
Comparative studies of the ca ...
Streptomyces hygroscopicus
Proteins
85
1146-1158
2017
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-
-
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1
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1
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3
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1
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1
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2
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1
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1
1
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748026
Hubrich
Chorismatase mechanisms revea ...
Streptomyces hygroscopicus subsp. ascomyceticus
J. Am. Chem. Soc.
137
11032-11037
2015
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1
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1
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2
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1
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1
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4
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1
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1
1
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725723
Juneja
Mechanistic implications for t ...
Streptomyces hygroscopicus subsp. ascomyceticus
J. Mol. Biol.
426
105-115
2014
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1
1
5
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1
1
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1
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2
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1
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1
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1
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1
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1
-
1
5
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1
1
1
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1
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1
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1
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732211
Hubrich
In vitro production and purifi ...
Streptomyces hygroscopicus
J. Biotechnol.
191
93-98
2014
-
-
1
-
-
-
-
-
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-
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1
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2
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1
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2
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1
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1
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1
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1
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2
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724541
Hubrich
Cinnamic acid derivatives as i ...
Streptomyces hygroscopicus subsp. ascomyceticus
Bioorg. Med. Chem. Lett.
23
1477-1481
2013
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1
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1
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1
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726377
Andexer
Biosynthesis of the immunosupp ...
Streptomyces hygroscopicus subsp. ascomyceticus
Proc. Natl. Acad. Sci. USA
108
4776-4781
2011
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1
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1
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3
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1
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