BRENDA - Enzyme Database
show all sequences of 3.3.2.13

Mechanistic implications for the chorismatase FkbO based on the crystal structure

Juneja, P.; Hubrich, F.; Diederichs, K.; Welte, W.; Andexer, J.N.; J. Mol. Biol. 426, 105-115 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21-RPpL1SL2(DE3) cells
Streptomyces hygroscopicus subsp. ascomyceticus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
in complex with 3-(2-carboxyethyl)benzoate, hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 20-25% (w/v) polyethylene glycol 4000
Streptomyces hygroscopicus subsp. ascomyceticus
Engineering
Protein Variants
Commentary
Organism
E338A
inactive
Streptomyces hygroscopicus subsp. ascomyceticus
E338Q
inactive
Streptomyces hygroscopicus subsp. ascomyceticus
F226A
the mutant shows 2.5% activity compared to the wild type enzyme
Streptomyces hygroscopicus subsp. ascomyceticus
R228A
the mutant shows 224.5% activity compared to the wild type enzyme
Streptomyces hygroscopicus subsp. ascomyceticus
Y215F
the mutant shows 12% activity compared to the wild type enzyme
Streptomyces hygroscopicus subsp. ascomyceticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-(2-carboxyethyl)benzoate
competitive inhibitor
Streptomyces hygroscopicus subsp. ascomyceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
chorismate
wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25C
Streptomyces hygroscopicus subsp. ascomyceticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
chorismate + H2O
Streptomyces hygroscopicus subsp. ascomyceticus
-
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces hygroscopicus subsp. ascomyceticus
Q9KID9
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Superdex 75 gel filtration
Streptomyces hygroscopicus subsp. ascomyceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
chorismate + H2O
-
725723
Streptomyces hygroscopicus subsp. ascomyceticus
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
fkbO
-
Streptomyces hygroscopicus subsp. ascomyceticus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.02
-
3-(2-carboxyethyl)benzoate
wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25C
Streptomyces hygroscopicus subsp. ascomyceticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-RPpL1SL2(DE3) cells
Streptomyces hygroscopicus subsp. ascomyceticus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with 3-(2-carboxyethyl)benzoate, hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 20-25% (w/v) polyethylene glycol 4000
Streptomyces hygroscopicus subsp. ascomyceticus
Engineering (protein specific)
Protein Variants
Commentary
Organism
E338A
inactive
Streptomyces hygroscopicus subsp. ascomyceticus
E338Q
inactive
Streptomyces hygroscopicus subsp. ascomyceticus
F226A
the mutant shows 2.5% activity compared to the wild type enzyme
Streptomyces hygroscopicus subsp. ascomyceticus
R228A
the mutant shows 224.5% activity compared to the wild type enzyme
Streptomyces hygroscopicus subsp. ascomyceticus
Y215F
the mutant shows 12% activity compared to the wild type enzyme
Streptomyces hygroscopicus subsp. ascomyceticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-(2-carboxyethyl)benzoate
competitive inhibitor
Streptomyces hygroscopicus subsp. ascomyceticus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.02
-
3-(2-carboxyethyl)benzoate
wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25C
Streptomyces hygroscopicus subsp. ascomyceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
chorismate
wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25C
Streptomyces hygroscopicus subsp. ascomyceticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
chorismate + H2O
Streptomyces hygroscopicus subsp. ascomyceticus
-
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Superdex 75 gel filtration
Streptomyces hygroscopicus subsp. ascomyceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
chorismate + H2O
-
725723
Streptomyces hygroscopicus subsp. ascomyceticus
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
-
-
?
Other publictions for EC 3.3.2.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753274
Zhang
How chorismatases regulate di ...
Streptomyces hygroscopicus
Chemistry
25
1326-1336
2019
-
-
-
-
2
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755348
Dong
Comparative studies of the ca ...
Streptomyces hygroscopicus
Proteins
85
1146-1158
2017
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748026
Hubrich
Chorismatase mechanisms revea ...
Streptomyces hygroscopicus subsp. ascomyceticus
J. Am. Chem. Soc.
137
11032-11037
2015
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725723
Juneja
Mechanistic implications for t ...
Streptomyces hygroscopicus subsp. ascomyceticus
J. Mol. Biol.
426
105-115
2014
-
-
1
1
5
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
5
-
-
1
1
1
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732211
Hubrich
In vitro production and purifi ...
Streptomyces hygroscopicus
J. Biotechnol.
191
93-98
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724541
Hubrich
Cinnamic acid derivatives as i ...
Streptomyces hygroscopicus subsp. ascomyceticus
Bioorg. Med. Chem. Lett.
23
1477-1481
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726377
Andexer
Biosynthesis of the immunosupp ...
Streptomyces hygroscopicus subsp. ascomyceticus
Proc. Natl. Acad. Sci. USA
108
4776-4781
2011
-
-
1
-
-
-
-
1
-
-
-
1
-
4
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-