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Literature summary for 3.3.2.10 extracted from

  • Lee, K.S.; Henriksen, N.M.; Ng, C.J.; Yang, J.; Jia, W.; Morisseau, C.; Andaya, A.; Gilson, M.K.; Hammock, B.D.
    Probing the orientation of inhibitor and epoxy-eicosatrienoic acid binding in the active site of soluble epoxide hydrolase (2017), Arch. Biochem. Biophys., 613, 1-11 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of human soluble epoxide hydrolase (sEH) in insect cells via baculovirus transfection system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-(1-isobutyrylpiperidin-4-yl)-3-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl)urea binding structure at the enzyme's active site, docking analysis and molecular dynamics simulations using the PDB ID 4JNC structure as template Homo sapiens
1-[4-methyl-6-(methylamino)-1,3,5-triazin-2-yl]-N-[2-(trifluoromethyl)benzyl]piperidine-4-carboxamide analysis of the crystal structure of enzyme-inhibitor complex Homo sapiens
N-[4-(2-methylpropanoyl)cyclohexyl]-N'-[4-(trifluoromethyl)phenyl]urea i.e. UC2389 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
14S,15R-epoxy-eicosatrienoic acid + H2O Homo sapiens
-
 14S,15R-dihydroxy-eicosatrienoic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P34913
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble epoxide hydrolase (sEH) from insect cells by affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,8Z)-N-((2-methoxy-4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl)sulfonyl)-10-(3-((Z)-oct-2-en-1-yl)oxiran-2-yl)deca-5,8-dienamide + H2O
-
 Homo sapiens ?
-
 ?
(5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid + H2O binding structure in the active site, docking analysis and molecular dynamics simulations Homo sapiens (5Z,8Z,11Z)-14,15-dihydroxyeicosa-5,8,11-trienoic acid
-
 ?
(5Z,8Z,11Z)-N-((2-methoxy-4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl) sulfonyl)-13-(3-pentyloxiran-2-yl)trideca-5,8,11-trienamide + H2O
-
 Homo sapiens ?
-
 ?
11,12-epoxy-eicosatrienoic acid + H2O
-
 Homo sapiens 11,12-dihydroxy-eicosatrienoic acid
-
 ?
14S,15R-epoxy-eicosatrienoic acid + H2O
-
 Homo sapiens 14S,15R-dihydroxy-eicosatrienoic acid
-
 ?
trans-1,3-diphenylpropene oxide + H2O
-
 Homo sapiens trans-1,3-diphenylpropane-1,2-diol
-
 ?

Synonyms

Synonyms Comment Organism
EPHX2
-
 Homo sapiens
SEH
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens