Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.3.2.10 extracted from

  • de Oliveira, G.; Adriani, P.; Borges, F.; Lopes, A.; Campana, P.; Chambergo, F.
    Epoxide hydrolase of Trichoderma reesei Biochemical properties and conformational characterization (2016), Int. J. Biol. Macromol., 89, 569-574 .
    View publication on PubMed
Search for more literature for 3.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 (S)-styrene oxide pH 7.0, 37°C Trichoderma reesei
21.8
-
 (R)-styrene oxide pH 7.0, 37°C Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38200
-
 calculated from sequence Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei G0R7E2
-
-
Trichoderma reesei QM9414 G0R7E2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-styrene oxide + H2O low activity, the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei (R)-styrene glycol
-
 ?
(R)-styrene oxide + H2O low activity, the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei QM9414 (R)-styrene glycol
-
 ?
(S)-styrene oxide + H2O the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei (S)-styrene glycol
-
 ?
(S)-styrene oxide + H2O the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei QM9414 (S)-styrene glycol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23 50 highly active at temperatures ranging from 23°C to 50°C Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 the enzyme conserves 80% of its activity for at least 20 min Trichoderma reesei
50
-
 activity decreases by 40% in the first 10 min, and 60% of the activity is preserved until 20 min of incubation Trichoderma reesei
70
-
 t1/2: 7.4 min Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
265
-
 (S)-styrene oxide pH 7.0, 37°C Trichoderma reesei
336
-
 (R)-styrene oxide pH 7.0, 37°C Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Trichoderma reesei

pH Range

pH Minimum pH Maximum Comment Organism
6 8.2 pH 6.0: about 55% of maximal activity, pH 8.2: about 40% of maximal activity Trichoderma reesei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.6
-
 (R)-styrene oxide pH 7.0, 37°C Trichoderma reesei
88.3
-
 (S)-styrene oxide pH 7.0, 37°C Trichoderma reesei