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Literature summary for 3.3.2.10 extracted from
- Development of a high-throughput screen for soluble epoxide hydrolase inhibition (2006), Anal. Biochem., 355, 71-80.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a high-throughput screen assay for soluble epoxide hydrolase inhibition using (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester as a substrate. Assay is accurate and precise | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-dicyclohexyl urea | - |
Homo sapiens |  |
| 1-cyclohexyl-3-dodecyl urea | - |
Homo sapiens | |
| 1-cyclohexyl-3-ethyl urea | - |
Homo sapiens | |
| 1-cyclohexyl-3-hexyl urea | - |
Homo sapiens | |
| 12-(3-adamantane-1-yl-ureido)-dodecanoic acid | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of a high-throughput screen assay for soluble epoxide hydrolase inhibition using (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester as a substrate. Assay is accurate and precise | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester + H2O | substrate for high-throughput screen | Homo sapiens | 6-methoxy-2-naphthaldehyde + ? | product is fluorescent | ? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | ratio kcat/Km value is 0.115 per s and microM | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000046 | - |
pH 7.0, 23°C | Homo sapiens | 12-(3-adamantane-1-yl-ureido)-dodecanoic acid | |
| 0.000019 | - |
pH 7.0, 23°C | Homo sapiens | 1-cyclohexyl-3-dodecyl urea | |
| 0.000029 | - |
pH 7.0, 23°C | Homo sapiens | 1-cyclohexyl-3-hexyl urea | |
| 0.000033 | - |
pH 7.0, 23°C | Homo sapiens | 1,3-dicyclohexyl urea | |
| 0.0098 | - |
pH 7.0, 23°C | Homo sapiens | 1-cyclohexyl-3-ethyl urea | |
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