Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tween-80 | when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate, the apparent activity of the epoxide hydrolase significantly increased by about 1.8fold | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | - |
Priestia megaterium | |
Ag+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
Al3+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
Cu2+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
Fe2+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
Fe3+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
Ni2+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
tetrahydrofuran | - |
Priestia megaterium | |
Zn2+ | 5 mM, more than 90% inhibition | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, activity is enhanced to 108% of control | Priestia megaterium | |
Mg2+ | 5 mM, activity is enhanced to 112% of control | Priestia megaterium | |
Mn2+ | 5 mM, activity is enhanced to 110% of control | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium ECU1001 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-glycidyl phenyl ether + H2O | the hydrolysis of (R)-epoxide in the presence of Tween-80 is 25.6 times faster than that of the (S)-epoxide | Priestia megaterium | ? | - |
? | |
(R)-glycidyl phenyl ether + H2O | the hydrolysis of (R)-epoxide in the presence of Tween-80 is 25.6 times faster than that of the (S)-epoxide | Priestia megaterium ECU1001 | ? | - |
? | |
2-methylphenyl glycidyl ether + H2O | - |
Priestia megaterium | ? | - |
? | |
2-methylphenyl glycidyl ether + H2O | - |
Priestia megaterium ECU1001 | ? | - |
? | |
4-methylphenylglycidyl ether + H2O | - |
Priestia megaterium | ? | - |
? | |
4-methylphenylglycidyl ether + H2O | - |
Priestia megaterium ECU1001 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Priestia megaterium |
40 | - |
when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate the temperature optimum shifts from 30°C to 40°C | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, no loss of activity | Priestia megaterium |
40 | - |
6 h, stable | Priestia megaterium |
50 | - |
when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate the half-life of the enzyme at 50°C increases by 2.5times | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | quite stable at | Priestia megaterium |