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Literature summary for 3.3.2.10 extracted from

  • Gomez, G.A.; Morisseau, C.; Hammock, B.D.; Christianson, D.W.
    Human soluble epoxide hydrolase: structural basis of inhibition by 4-(3-cyclohexylureido)-carboxylic acids (2006), Protein Sci., 15, 58-64.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine enzyme inhibition is used in treatment of hypertension Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expression in insect cells using the baculovirus infection system Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in complex with inhibitors 4-(3-cyclohexylureido)-ethanoic acid, N-cyclohexyl-N'-iodophenyl urea, 4-(3-cyclohexylureido)-butyric acid, 4-(3-cyclohexylureido)-hexanoic acid, and 4-(3-cyclohexylureido)-heptanoic acid, vapour diffusion method, 0.005 ml sitting drops of protein solution containing 12-16 mg/ml enzyme, 3 mM DTT, 0.1 M sodium phosphate, pH 7.4, mixed with 0.005 ml precipitant solution containing 0.1 M Tris, pH 8.4, and 34% w/v PEG3350, and 0.005 ml n-hexadecyl-beta-D-maltoside solution, versus 1 ml reservoir solution, 4°C, 7 days, soaking of crystals in precipitation solution containing 30 mM inhibitor, 3 days, 4°C, cryoprotection by 20% sucrose, X-ray diffraction structure determination and analysis at 2.3-3.0 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-(3-adamantan-1-yl-ureido) butyric acid
-
Homo sapiens
4-(3-adamantan-1-yl-ureido) butyric acid methyl ester
-
Homo sapiens
4-(3-cyclohexylureido)-butyric acid binding structure analysis Homo sapiens
4-(3-cyclohexylureido)-butyric acid methyl ester
-
Homo sapiens
4-(3-cyclohexylureido)-heptanoic acid binding structure analysis Homo sapiens
4-(3-cyclohexylureido)-hexanoic acid binding structure analysis Homo sapiens
4-(3-cyclohexylureido)-hexanoic acid methyl ester
-
Homo sapiens
methyl N-(cyclohexylcarbamoyl)glycinate
-
Homo sapiens
N,N'-diadamantyl urea
-
Homo sapiens
N-(cyclohexylcarbamoyl)glycine binding structure analysis Homo sapiens
N-cyclohexyl-N'-(3-phenyl)propyl urea
-
Homo sapiens
N-cyclohexyl-N'-iodophenyl urea binding structure analysis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P34913
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from insect cells in several steps including a final gel filtration step Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-14,15-epoxyeicosatrienoic acid + H2O simulation of substrate binding, modeling Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
SEH
-
Homo sapiens