Application | Comment | Organism |
---|---|---|
medicine | enzyme inhibition is used in treatment of hypertension | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
enzyme expression in insect cells using the baculovirus infection system | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with inhibitors 4-(3-cyclohexylureido)-ethanoic acid, N-cyclohexyl-N'-iodophenyl urea, 4-(3-cyclohexylureido)-butyric acid, 4-(3-cyclohexylureido)-hexanoic acid, and 4-(3-cyclohexylureido)-heptanoic acid, vapour diffusion method, 0.005 ml sitting drops of protein solution containing 12-16 mg/ml enzyme, 3 mM DTT, 0.1 M sodium phosphate, pH 7.4, mixed with 0.005 ml precipitant solution containing 0.1 M Tris, pH 8.4, and 34% w/v PEG3350, and 0.005 ml n-hexadecyl-beta-D-maltoside solution, versus 1 ml reservoir solution, 4°C, 7 days, soaking of crystals in precipitation solution containing 30 mM inhibitor, 3 days, 4°C, cryoprotection by 20% sucrose, X-ray diffraction structure determination and analysis at 2.3-3.0 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(3-adamantan-1-yl-ureido) butyric acid | - |
Homo sapiens | |
4-(3-adamantan-1-yl-ureido) butyric acid methyl ester | - |
Homo sapiens | |
4-(3-cyclohexylureido)-butyric acid | binding structure analysis | Homo sapiens | |
4-(3-cyclohexylureido)-butyric acid methyl ester | - |
Homo sapiens | |
4-(3-cyclohexylureido)-heptanoic acid | binding structure analysis | Homo sapiens | |
4-(3-cyclohexylureido)-hexanoic acid | binding structure analysis | Homo sapiens | |
4-(3-cyclohexylureido)-hexanoic acid methyl ester | - |
Homo sapiens | |
methyl N-(cyclohexylcarbamoyl)glycinate | - |
Homo sapiens | |
N,N'-diadamantyl urea | - |
Homo sapiens | |
N-(cyclohexylcarbamoyl)glycine | binding structure analysis | Homo sapiens | |
N-cyclohexyl-N'-(3-phenyl)propyl urea | - |
Homo sapiens | |
N-cyclohexyl-N'-iodophenyl urea | binding structure analysis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34913 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from insect cells in several steps including a final gel filtration step | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-14,15-epoxyeicosatrienoic acid + H2O | simulation of substrate binding, modeling | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SEH | - |
Homo sapiens |