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Literature summary for 3.3.2.10 extracted from

  • Arahira, M.; Nong, V.H.; Udaka, K.; Fukazawa, C.
    Purification, molecular cloning and ethylene-inducible expression of a soluble-type epoxide hydrolase from soybean (Glycine max [L.] Merr.) (2000), Eur. J. Biochem., 267, 2649-2657.
    View publication on PubMed
Search for more literature for 3.3.2.10

Activating Compound

Activating Compound Comment Organism Structure
additional information ethylene induces the enzyme expression in stem and seeds, not in leaves Glycine max

Cloned(Commentary)

Cloned (Comment) Organism
cDNA library construction, DNA and amino acid sequence determination of the gene encoding the enzyme, genetic organization in a small EH gene family in soybean, overexpression in Escherichia coli strain BL21(DE3) Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Glycine max
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis Glycine max
36000
-
 1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis Glycine max
36171
-
 1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycine max the soluble-type epoxide hydrolase may play a crucial role in the self-defense system of the plant ?
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max O49857
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from dry mature seeds by ammonium sulfate fractionation, ion exchange chromatography and chromatofocusing, the latter step allows separation from the co-purifying seed-specific PR-8 chitinase Chib2, recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 55°C for 10 min, chromatofocusing and gel filtration Glycine max

Reaction

Reaction Comment Organism Reaction ID
an epoxide + H2O = a glycol the catalytic triad is formed by Asp126, Asp285, and His320 Glycine max
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information nearly no expression in leaves Glycine max
-
seed mature, dry seeds, high enzyme expression level at the very early stage of seed development 13 days after flowering, the expression decreases gradually duting seed development and is nearly ceased at the very late stage 58 days after flowering Glycine max
-
stem
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the soluble-type epoxide hydrolase may play a crucial role in the self-defense system of the plant Glycine max ?
-
 ?
styrene oxide + H2O
-
 Glycine max ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, recombinant enzyme, SDS-PAGE, 1 * 36171, recombinant and native enzymes, mass spectrometry, 1 * 36000, native enzyme, sedimentation equilibrium analysis Glycine max
More the enzyme contains an N-terminal transit peptide Glycine max

Synonyms

Synonyms Comment Organism
soluble-type epoxide hydrolase
-
 Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max isoelectric focusing
-
 5.2