Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | maximal activity with dimethyl formamide as co-solvent, effects of solvents on enzyme activity, overview | Pseudomonas sp. |
Application | Comment | Organism |
---|---|---|
synthesis | the enantioselective enzyme is useful in production of chiral substances, e.g. production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat by whole cells of Pseudomonas sp. strain BZS21, maximal activity with dimethyl formamide as co-solvent | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. BZS21 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:styrene oxide-grown cell | - |
Pseudomonas sp. | - |
culture condition:styrene-grown cell | - |
Pseudomonas sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
racemic ethyl 3-phenylglycidate + H2O | production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat | Pseudomonas sp. | ? | - |
? | |
racemic ethyl 3-phenylglycidate + H2O | production of (2R,3S)-ethyl 3-phenylglycidate with 95% enantiomeric excess and 26% yield in 12 h from 0.2% (w/v) of the racemat | Pseudomonas sp. BZS21 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas sp. |