Literature summary for 3.3.2.10 extracted from

Gomez, G.A.; Morisseau, C.; Hammock, B.D.; Christianson, D.W.
Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis (2004), Biochemistry, 43, 4716-4723.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme in complex with N-cyclohexyl-N'-(iodophenyl)urea, sitting drop vapour diffusion method, 0.005 ml of 12-16 mg/ml protein in 3 mM DTT, 0.1 M sodium phosphate, pH 7.4, is mixed with 0.005 ml precipitation solution containing 0.1 M Tris, pH 9.0, 30% w/v PEG 4000, and 0.2 M Li2SO4, versus 1 ml reservoir of precipitation solution, 4°C, 10 days, soaking of crystals in inhibitor solution and n-hexadecyl-beta-D-maltoside, X-ray diffraction structure determination and analysis at 2.35 A resolution, modeling	Homo sapiens
sitting drop vapour diffusion method, X-ray crystal structure determined at 2.6 A resolution, structure of the complex with the inhibitor N-cyclohexyl-N-(iodophenyl)urea determine at 2.35 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant enzyme in complex with N-cyclohexyl-N'-(iodophenyl)urea, sitting drop vapour diffusion method, 0.005 ml of 12-16 mg/ml protein in 3 mM DTT, 0.1 M sodium phosphate, pH 7.4, is mixed with 0.005 ml precipitation solution containing 0.1 M Tris, pH 9.0, 30% w/v PEG 4000, and 0.2 M Li2SO4, versus 1 ml reservoir of precipitation solution, 4°C, 10 days, soaking of crystals in inhibitor solution and n-hexadecyl-beta-D-maltoside, X-ray diffraction structure determination and analysis at 2.35 A resolution, modeling

Homo sapiens

sitting drop vapour diffusion method, X-ray crystal structure determined at 2.6 A resolution, structure of the complex with the inhibitor N-cyclohexyl-N-(iodophenyl)urea determine at 2.35 A resolution

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
N-cyclohexyl-N'-(iodophenyl)urea	binding structure at the C-terminus	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P34913	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
an epoxide + H2O = a glycol	the C-terminal part harbors the epoxide hydrolase activity, the phosphatase activity of the enzyme is located at the N-terminal part of EC 3.1.3.76, both catalytic sites act independently, the epoxide hydrolase reaction proceeds though an alkyl-enzyme intermediate involving the catalytic Asp333, and hydrogen bonds with Tyr381 and Tyr465, reaction mechanism, structure-mechanism relationship	Homo sapiens	a

Subunits

Subunits	Comment	Organism
More	the 25 kDa C-terminal part, with a hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity, the enzyme's phosphatase activity of EC3.1.3.76 is located at the 35 kDa N-terminal part which has a different alpha/beta-fold, quarternary structure analysis, structure-mechanism relationship	Homo sapiens

Synonyms

Synonyms	Comment	Organism
More	see also EC 3.1.3.76	Homo sapiens