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Literature summary for 3.3.2.10 extracted from
- Properties of cytosolic epoxide hydrolase purified from the liver of untreated and clofibrate-treated mice. Characterization of optimal assay conditions, substrate specificity and effects of modulators on the catalytic activity (1985), Eur. J. Biochem., 150, 7-16.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Aminotriazole | slight activation | Mus musculus |  |
| benzil | - |
Mus musculus | |
| Clofibrate | slight inhibition of enzyme from untreated mice, slight activation of the enzyme from clofibrate-treated mice | Mus musculus | |
| ellipticine | - |
Mus musculus | |
| Harmane | - |
Mus musculus | |
| iodoacetamide | - |
Mus musculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Phenylchalcone oxide | - |
Mus musculus | |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Mus musculus | |
| alpha-naphthoflavone | - |
Mus musculus | |
| beta-naphthoflavone | - |
Mus musculus | |
| Chalcone oxide | - |
Mus musculus | |
| Clofibrate | slight inhibition of enzyme from untreated mice, slight activation of the enzyme from clofibrate-treated mice | Mus musculus | |
| Dimethylsulfoxide | - |
Mus musculus | |
| NEM | - |
Mus musculus | |
| omega-bromo-4-nitroacetophenone | - |
Mus musculus | |
| p-Hydroxymercuriphenylsulfonate | - |
Mus musculus | |
| tetrahydrofuran | - |
Mus musculus | |
| trichloropropylene oxide | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0042 | - |
trans-stilbene oxide | enzyme from clofibrate-treated animals | Mus musculus | |
| 0.0049 | - |
trans-stilbene oxide | enzyme from untreated animals | Mus musculus | |
| 0.066 | - |
cis-stilbene oxide | enzyme from clofibrate-treated animals | Mus musculus | |
| 0.073 | - |
cis-stilbene oxide | enzyme from untreated animals | Mus musculus | |
| 1.36 | - |
styrene oxide | enzyme from clofibbrate-treated animals | Mus musculus | |
| 1.98 | - |
styrene oxide | enzyme from untreated animals | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
untreated and clofibrate-treated animals | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to oxygen | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 6 months | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-stilbene oxide + H2O | - |
Mus musculus | ? | - |
? | |
| cyclohexene oxide + H2O | - |
Mus musculus | ? | - |
? | |
| styrene oxide + H2O | - |
Mus musculus | styrene glycol | - |
? | |
| trans-beta-propylstyrene oxide + H2O | - |
Mus musculus | ? | - |
? | |
| trans-ethyl styrene oxide + H2O | - |
Mus musculus | ? | - |
? | |
| trans-stilbene oxide + H2O | - |
Mus musculus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Mus musculus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 55 | 37°C: about 60% of maximal activity, 55°C: about 50% of maximal activity of the enzyme from clofibrate-treated animals, about 80% of maximal activity of the enzyme from untreated animals | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.4 | - |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
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