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Literature summary for 3.3.2.1 extracted from
- Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain (2006), Chem. Biol., 13, 409-419.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pGEM7z and the pET15bTEV vector for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystal structure of the EntB protein is determined at a resolution of 2.3 A | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D227R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| D240R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| D244R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| D263R | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| F264E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| I239D | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| K269E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| M249E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| R247E | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
| S245A | mutant constructed for functional analysis of EntB-EntE interaction | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33090 | - |
calculated molecular mass | Escherichia coli |
| 33100 | - |
determinecd by MALDI-TOF spectrometry | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0ADI4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification is obtained by two metal chelate chromatography steps seperated by emoval of the His tag with TEV protease | Escherichia coli |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EntB | contains an N-terminal isochorismate lyase domain | Escherichia coli |
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