Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+/-)-3-[(carboxyethenyl)oxy]-6-hydroxy-1-benzoic acid | i.e. 5-(1-carboxyvinyloxy)-2-hydroxybenzoic acid | Escherichia coli | |
3-[(carboxyethenyl)oxy]benzoic acid | i.e. 3-(1-carboxyvinyloxy)benzoic acid | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
isochorismate | - |
Escherichia coli | |
0.023 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
0.086 | - |
(+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
0.12 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate | - |
Escherichia coli | |
0.28 | - |
(+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
35 | - |
chorismate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
5 * 35000, SDS-PAGE | Escherichia coli |
159000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate + H2O | Escherichia coli | biosynthesis of enterobactin | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 25 mM Tris buffer, pH 8.0, 5 mM dithiothreitol, 50% glycerol, stable for several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid + H2O | - |
Escherichia coli | 3,4-dihydroxy-cyclohex-1-enecarboxylic acid + pyruvate | - |
? | |
(+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate + H2O | i.e. (S)-3-(1-carboxyvinyloxy)-cyclohepta-1,6-dienecarboxylic acid | Escherichia coli | (S)-3-hydroxy-cyclohepta-1,6-dienecarboxylic acid + pyruvate | - |
? | |
(+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid + H2O | - |
Escherichia coli | (S)-3-hydroxycyclohex-1-enecarboxylic acid + pyruvate | - |
? | |
(+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid + H2O | - |
Escherichia coli | (R)-5-hydroxycyclohex-3-enecarboxylic acid + pyruvate | - |
? | |
chorismate + H2O | slow hydrolysis | Escherichia coli | 3,4-dihydroxy-cyclohexa-1,5-dienecarboxylic acid + pyruvate | - |
? | |
isochorismate + H2O | - |
Escherichia coli | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
isochorismate + H2O | biosynthesis of enterobactin | Escherichia coli | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * 35000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.17 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
9 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate | - |
Escherichia coli | |
10 | - |
isochorismate | - |
Escherichia coli | |
16.3 | - |
(+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
21.7 | - |
(+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
217 | - |
chorismate | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Escherichia coli |