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Literature summary for 3.2.2.B6 extracted from

  • McPherson, R.L.; Abraham, R.; Sreekumar, E.; Ong, S.E.; Cheng, S.J.; Baxter, V.K.; Kistemaker, H.A.; Filippov, D.V.; Griffin, D.E.; Leung, A.K.
    ADP-ribosylhydrolase activity of Chikungunya virus macrodomain is critical for virus replication and virulence (2017), Proc. Natl. Acad. Sci. USA, 114, 1666-1671 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.B6

Protein Variants

Protein Variants Comment Organism
D10A 16% residual hydrolase activity Chikungunya virus
G112E 3.7% residual hydrolase activity Chikungunya virus
G32A 75% residual hydrolase activity Chikungunya virus
G32E 3.8% residual hydrolase activity Chikungunya virus
G32E/V113R/Y114N 15% residual hydrolase activity Chikungunya virus
G32S 48% residual hydrolase activity Chikungunya virus
R144A 96% residual hydrolase activity Chikungunya virus
T111A 64.1% residual hydrolase activity Chikungunya virus
Y114A 40.6% residual hydrolase activity Chikungunya virus

Organism

Organism UniProt Comment Textmining
Chikungunya virus Q8JUX6
-
-
Chikungunya virus S27 - African prototype Q8JUX6
-
-

Reaction

Reaction Comment Organism Reaction ID
protein-Nomega-(ADP-D-ribosyl)-L-arginine + H2O = ADP-D-ribose + protein-L-arginine (1) Chikungunya virus
a
protein-Nomega-(ADP-D-ribosyl)-L-glutamate + H2O = ADP-D-ribose + protein-L-glutamate (2) Chikungunya virus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is able to hydrolyze ADP-ribose groups from mono(ADP-ribosyl)ated proteins. Its substrate specificity is mono(ADP-ribosyl)ated aspartate and glutamate but not lysine residues Chikungunya virus ?
-
 ?
additional information enzyme is able to hydrolyze ADP-ribose groups from mono(ADP-ribosyl)ated proteins. Its substrate specificity is mono(ADP-ribosyl)ated aspartate and glutamate but not lysine residues Chikungunya virus S27 - African prototype ?
-
 ?
PARP10-Nomega-(ADP-D-ribosyl)-L-arginine + H2O substrate is the catalytic domain of protein PARP10 Chikungunya virus ADP-D-ribose + PARP10-L-arginine
-
 ?
PARP10-Nomega-(ADP-D-ribosyl)-L-arginine + H2O substrate is the catalytic domain of protein PARP10 Chikungunya virus S27 - African prototype ADP-D-ribose + PARP10-L-arginine
-
 ?

Synonyms

Synonyms Comment Organism
nonstructural protein 3
-
 Chikungunya virus
Polyprotein P1234
-
 Chikungunya virus

General Information

General Information Comment Organism
physiological function mutant viruses lacking hydrolase activity are unable to replicate in mammalian BHK-21 cells or mosquito Aedes albopictus cells and rapidly revert catalytically inactivating mutations. Mutants with reduced enzymatic activity have slower replication in mammalian neuronal cells and reduced virulence in 2-day-old mice Chikungunya virus