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Literature summary for 3.2.2.9 extracted from

  • Siu, K.K.; Lee, J.E.; Smith, G.D.; Horvatin-Mrakovcic, C.; Howell, P.L.
    Structure of Staphylococcus aureus 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (2008), Acta Crystallogr. Sect. F, 64, 343-350.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 Staphylococcus aureus
in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00252
-
 S-adenosyl-L-homocysteine recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 Staphylococcus aureus
0.0053
-
 S-adenosyl-L-homocysteine recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AF14
-
-
Staphylococcus aureus Q99TQ0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration Staphylococcus aureus
Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + H2O
-
 Staphylococcus aureus adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?

Subunits

Subunits Comment Organism
dimer biologically active dimer, X-ray crystallography Staphylococcus aureus

Synonyms

Synonyms Comment Organism
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Staphylococcus aureus
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Escherichia coli
MTAN MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine Staphylococcus aureus
MTAN MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00873
-
 S-adenosyl-L-homocysteine recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 Staphylococcus aureus
0.45
-
 S-adenosyl-L-homocysteine recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 Escherichia coli