Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.9 extracted from

  • Siu, K.K.; Lee, J.E.; Sufrin, J.R.; Moffatt, B.A.; McMillan, M.; Cornell, K.A.; Isom, C.; Howell, P.L.
    Molecular determinants of substrate specificity in plant 5-methylthioadenosine nucleosidases (2008), J. Mol. Biol., 378, 112-128.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Codon+ cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 5'-methylthioadenosine in 100 mM sodium phosphate buffer, pH 7.0 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27462
-
 x * 27462, isoform MATN2, calculated from amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Arabidopsis thaliana
-
 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q9T0I8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
 using S-adenosyl-L-homocysteine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-butylthioadenosine + H2O 24% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + 5-butylthio-D-ribose
-
 ?
5'-chloroadenosine + H2O 13% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + 5-chloro-D-ribose
-
 ?
5'-ethylthioadenosine + H2O 42% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + 5-ethylthio-D-ribose
-
 ?
5'-iodoadenosine + H2O 17% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + 5-iodo-D-ribose
-
 ?
5'-isobutylthioadenosine + H2O 32% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + 5-isobutylthio-D-ribose
-
 ?
5'-methylthioadenosine + H2O 100% activity Arabidopsis thaliana 5-methylthio-D-ribose + adenine
-
 ?
5'-phenylthioadenosine + H2O 39% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + 5-phenylthio-D-ribose
-
 ?
adenosine + H2O 6% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + D-ribose
-
 ?
S-adenosyl-L-homocysteine + H2O 14% activity compared to 5'-methylthioadenosine Arabidopsis thaliana adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Arabidopsis thaliana S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?

Subunits

Subunits Comment Organism
? x * 27462, isoform MATN2, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Arabidopsis thaliana
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2
-
 Arabidopsis thaliana
MTA/SAH nucleosidase
-
 Arabidopsis thaliana
MTAN2
-
 Arabidopsis thaliana
MTAN2 isoform Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 5'-methylthioadenosine in 100 mM sodium phosphate buffer, pH 7.0 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Arabidopsis thaliana