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Literature summary for 3.2.2.9 extracted from

  • Ferro, A.J.; Barrett, A.; Shapiro, S.K.
    Kinetic properties and the effect of substrate analogues on 5'-methylthioadenosine nucleosidase from Escherichia coli (1976), Biochim. Biophys. Acta, 438, 487-494.
    View publication on PubMed
Search for more literature for 3.2.2.9

Inhibitors

Inhibitors Comment Organism Structure
5'-ethylthioadenosine competitive inhibition Escherichia coli
5'-n-Propylthioadenosine competitive inhibition Escherichia coli
adenine
-
 Escherichia coli
S-adenosylhomocysteine competitive inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00031
-
 5'-methylthioadenosine
-
 Escherichia coli
0.3
-
 5'-methylthioadenosine
-
 Rattus rattus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-methylthioadenosine + H2O Escherichia coli
-
 ?
-
 ?
5'-methylthioadenosine + H2O Escherichia coli B / ATCC 11303
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Escherichia coli rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 35% ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-
Rattus rattus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
220-fold Escherichia coli
30-fold Rattus rattus

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland ventral lobe
-
 Rattus rattus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.93
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°, repeatedly frozen and thawed without loss of activity over a 9 months period, upon storage at 4°C only 50% of activity retained after 3 weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-ethylthioadenosine + H2O rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 118% Escherichia coli adenine + 5-ethylthio-D-ribose
-
 ?
5'-ethylthioadenosine + H2O rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 118% Escherichia coli B / ATCC 11303 adenine + 5-ethylthio-D-ribose
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Rattus rattus 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli B / ATCC 11303 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli ?
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli B / ATCC 11303 ?
-
 ?
5'-n-propylthioadenosine + H2O rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 106% Escherichia coli adenine + 5-n-propylthioribose
-
 ?
5'-n-propylthioadenosine + H2O rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 106% Escherichia coli B / ATCC 11303 adenine + 5-n-propylthioribose
-
 ?
S-adenosyl-L-homocysteine + H2O rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 35% Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 35% Escherichia coli B / ATCC 11303 adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 35% Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine nucleosidase
-
 Escherichia coli