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Literature summary for 3.2.2.8 extracted from

  • Giabbai, B.; Degano, M.
    Crystal structure to 1.7 ANG of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy (2004), Structure, 12, 739-749.
    View publication on PubMed
Search for more literature for 3.2.2.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Escherichia coli

Protein Variants

Protein Variants Comment Organism
H239A dramatic increase in Km for uridine, unchanged kcat Escherichia coli
H82N small increase in Km, increase in kcat Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.128
-
 5-fluorouridine pH 7.3, 37°C Escherichia coli
0.142
-
 uridine pH 7.3, 37°C Escherichia coli
0.186
-
 5-Bromouridine pH 7.3, 37°C Escherichia coli
0.22
-
 5-iodouridine pH 7.3, 37°C Escherichia coli
0.329
-
 5-methyluridine pH 7.3, 37°C Escherichia coli
0.345
-
 uridine pH 7.3, 37°C, H82N mutant Escherichia coli
0.532
-
 cytidine pH 7.3, 37°C Escherichia coli
1.9
-
 uridine pH 7.3, 37°C, H239A mutant Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 homotetramer, 4 * 33000, SDS-PAGE Escherichia coli
137000
-
 calculated from amino acid sequence Escherichia coli
143000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromouridine + H2O
-
 Escherichia coli 5-bromouracil + D-ribose
-
 ?
5-fluorouridine + H2O
-
 Escherichia coli 5-fluorouracil + D-ribose
-
 ?
5-iodouridine + H2O
-
 Escherichia coli 5-iodouracil + D-ribose
-
 ?
5-methyluridine + H2O
-
 Escherichia coli 5-methyluracil + D-ribose
-
 ?
cytidine + H2O
-
 Escherichia coli cytosine + D-ribose
-
 ?
additional information no substrate: deazouridine Escherichia coli ?
-
 ?
uridine + H2O
-
 Escherichia coli uracil + D-ribose
-
 ?

Subunits

Subunits Comment Organism
tetramer homotetramer, 4 * 33000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
YeiK
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 uridine pH 7.3, 37°C, H239A mutant Escherichia coli
4.7
-
 uridine pH 7.3, 37°C Escherichia coli
11.6
-
 cytidine pH 7.3, 37°C Escherichia coli
14.7
-
 5-fluorouridine pH 7.3, 37°C Escherichia coli
15.5
-
 uridine pH 7.3, 37°C, H82N mutant Escherichia coli
25.5
-
 5-methyluridine pH 7.3, 37°C Escherichia coli
30.8
-
 5-Bromouridine pH 7.3, 37°C Escherichia coli
42.7
-
 5-iodouridine pH 7.3, 37°C Escherichia coli