BRENDA - Enzyme Database show
show all sequences of 3.2.2.8

Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens

Terada, M.; Tatibana, M.; Hayaishi, O.; J. Biol. Chem. 242, 5578-5585 (1967)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
adenosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
AgCl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
CaCl2
at 1 mM, inhibition less than 10%
Pseudomonas fluorescens
CdCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CoCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CuCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
cytidine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
EDTA
at 1 mM: inhibits uridine hydrolysis by 50%
Pseudomonas fluorescens
FeCl3
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
FeSO4
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
HgCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
Inosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
MgCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
MnSO4
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
NH4Cl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
ZnCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
Inosine
pH 8.5
Pseudomonas fluorescens
0.5
-
adenosine
pH 8.5
Pseudomonas fluorescens
0.5
-
Inosine
-
Pseudomonas fluorescens
0.6
-
guanosine
-
Pseudomonas fluorescens
0.8
-
uridine
-
Pseudomonas fluorescens
1
-
cytidine
pH 8.5
Pseudomonas fluorescens
2.5
-
5-Bromouridine
-
Pseudomonas fluorescens
6.5
-
Xanthosine
-
Pseudomonas fluorescens
7.5
-
1-beta-D-ribofuranosylthymine
-
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-D-ribosylpyrimidine
Pseudomonas fluorescens
-
pyrimidine + D-ribose
-
Pseudomonas fluorescens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas fluorescens
-
-
-
Purification (Commentary)
Commentary
Organism
partial purification, 300fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
272
-
at 37°C
Pseudomonas fluorescens
Storage Stability
Storage Stability
Organism
-15°C, 1-3 mg of protein per ml, stable for 6 months
Pseudomonas fluorescens
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month
Pseudomonas fluorescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-beta-D-ribofuranosylnicotinamide + H2O
-
137117
Pseudomonas fluorescens
nicotinamide + D-ribose
-
137117
Pseudomonas fluorescens
?
1-beta-D-ribofuranosylthymine + H2O
-
137117
Pseudomonas fluorescens
D-ribose + thymine
-
137117
Pseudomonas fluorescens
?
4-amino-5-imidazolecarboxamide ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
4-amino-5-imidazolecarboxamide + D-ribose
-
137117
Pseudomonas fluorescens
?
5-bromouridine + H2O
-
137117
Pseudomonas fluorescens
5-bromouracil + D-ribose
-
137117
Pseudomonas fluorescens
?
6-azauridine + H2O
poor substrate
137117
Pseudomonas fluorescens
6-azauracil + D-ribose
-
137117
Pseudomonas fluorescens
?
adenosine + H2O
-
137117
Pseudomonas fluorescens
adenine + D-ribose
-
137117
Pseudomonas fluorescens
?
cytidine + H2O
-
137117
Pseudomonas fluorescens
cytosine + D-ribose
-
137117
Pseudomonas fluorescens
?
guanosine + H2O
-
137117
Pseudomonas fluorescens
guanine + D-ribose
-
137117
Pseudomonas fluorescens
?
imidazoleacetic acid ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
imidazoleacetic acid + D-ribose
-
137117
Pseudomonas fluorescens
?
inosine + H2O
-
137117
Pseudomonas fluorescens
hypoxanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine + H2O
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
purine D-ribonucleosides + H2O
prefers pyrimidines
137117
Pseudomonas fluorescens
purine + D-ribose
-
137117
Pseudomonas fluorescens
?
tubercidin + H2O
poor substrate
137117
Pseudomonas fluorescens
1-deazaadenine + D-ribose
-
137117
Pseudomonas fluorescens
?
uridine + H2O
-
137117
Pseudomonas fluorescens
uracil + D-ribose
-
137117
Pseudomonas fluorescens
?
xanthosine + H2O
-
137117
Pseudomonas fluorescens
xanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
for 10 min at pH 5.0, 50% of activity remains; for 10 min at pH 7.0-9.2, more than 90% of activity remains
Pseudomonas fluorescens
60
-
for 15 min, 60% denaturation
Pseudomonas fluorescens
70
-
for 5 min at pH 8.0, complete inactivation
Pseudomonas fluorescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
substrate: inosine
Pseudomonas fluorescens
6.5
-
substrate: guanosine
Pseudomonas fluorescens
7
-
substrate: xanthosine
Pseudomonas fluorescens
8
-
substrate: 1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
8.5
-
substrate: cytidine, uridine, 5-bromouridine and adenosine
Pseudomonas fluorescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
adenosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
AgCl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
CaCl2
at 1 mM, inhibition less than 10%
Pseudomonas fluorescens
CdCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CoCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CuCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
cytidine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
EDTA
at 1 mM: inhibits uridine hydrolysis by 50%
Pseudomonas fluorescens
FeCl3
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
FeSO4
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
HgCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
Inosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
MgCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
MnSO4
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
NH4Cl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
ZnCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
Inosine
pH 8.5
Pseudomonas fluorescens
0.5
-
adenosine
pH 8.5
Pseudomonas fluorescens
0.5
-
Inosine
-
Pseudomonas fluorescens
0.6
-
guanosine
-
Pseudomonas fluorescens
0.8
-
uridine
-
Pseudomonas fluorescens
1
-
cytidine
pH 8.5
Pseudomonas fluorescens
2.5
-
5-Bromouridine
-
Pseudomonas fluorescens
6.5
-
Xanthosine
-
Pseudomonas fluorescens
7.5
-
1-beta-D-ribofuranosylthymine
-
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-D-ribosylpyrimidine
Pseudomonas fluorescens
-
pyrimidine + D-ribose
-
Pseudomonas fluorescens
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification, 300fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
272
-
at 37°C
Pseudomonas fluorescens
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, 1-3 mg of protein per ml, stable for 6 months
Pseudomonas fluorescens
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-beta-D-ribofuranosylnicotinamide + H2O
-
137117
Pseudomonas fluorescens
nicotinamide + D-ribose
-
137117
Pseudomonas fluorescens
?
1-beta-D-ribofuranosylthymine + H2O
-
137117
Pseudomonas fluorescens
D-ribose + thymine
-
137117
Pseudomonas fluorescens
?
4-amino-5-imidazolecarboxamide ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
4-amino-5-imidazolecarboxamide + D-ribose
-
137117
Pseudomonas fluorescens
?
5-bromouridine + H2O
-
137117
Pseudomonas fluorescens
5-bromouracil + D-ribose
-
137117
Pseudomonas fluorescens
?
6-azauridine + H2O
poor substrate
137117
Pseudomonas fluorescens
6-azauracil + D-ribose
-
137117
Pseudomonas fluorescens
?
adenosine + H2O
-
137117
Pseudomonas fluorescens
adenine + D-ribose
-
137117
Pseudomonas fluorescens
?
cytidine + H2O
-
137117
Pseudomonas fluorescens
cytosine + D-ribose
-
137117
Pseudomonas fluorescens
?
guanosine + H2O
-
137117
Pseudomonas fluorescens
guanine + D-ribose
-
137117
Pseudomonas fluorescens
?
imidazoleacetic acid ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
imidazoleacetic acid + D-ribose
-
137117
Pseudomonas fluorescens
?
inosine + H2O
-
137117
Pseudomonas fluorescens
hypoxanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine + H2O
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
purine D-ribonucleosides + H2O
prefers pyrimidines
137117
Pseudomonas fluorescens
purine + D-ribose
-
137117
Pseudomonas fluorescens
?
tubercidin + H2O
poor substrate
137117
Pseudomonas fluorescens
1-deazaadenine + D-ribose
-
137117
Pseudomonas fluorescens
?
uridine + H2O
-
137117
Pseudomonas fluorescens
uracil + D-ribose
-
137117
Pseudomonas fluorescens
?
xanthosine + H2O
-
137117
Pseudomonas fluorescens
xanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
for 10 min at pH 5.0, 50% of activity remains; for 10 min at pH 7.0-9.2, more than 90% of activity remains
Pseudomonas fluorescens
60
-
for 15 min, 60% denaturation
Pseudomonas fluorescens
70
-
for 5 min at pH 8.0, complete inactivation
Pseudomonas fluorescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
substrate: inosine
Pseudomonas fluorescens
6.5
-
substrate: guanosine
Pseudomonas fluorescens
7
-
substrate: xanthosine
Pseudomonas fluorescens
8
-
substrate: 1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
8.5
-
substrate: cytidine, uridine, 5-bromouridine and adenosine
Pseudomonas fluorescens
Other publictions for EC 3.2.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751203
Lenz
Structural explanation for th ...
Escherichia coli
J. Comput. Aided Mol. Des.
32
1375-1388
2018
-
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1
1
1
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3
-
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1
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1
1
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751391
Fan
QM/MM and MM MD Simulations o ...
Escherichia coli
J. Phys. Chem. B
122
1121-1131
2018
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1
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752168
Dalberto
-
Thermodynamics, functional an ...
Leishmania braziliensis
RSC Adv.
7
48861-48875
2017
-
-
1
1
-
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-
5
-
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1
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1
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6
1
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5
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1
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1
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5
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1
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6
1
-
-
-
5
-
-
-
-
-
-
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-
5
5
724342
Minici
New determinants in the cataly ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochemistry
51
4590-4599
2012
-
-
1
1
-
-
-
-
-
1
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2
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1
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2
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1
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1
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1
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1
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2
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726441
Porcelli
Thermal unfolding of nucleosid ...
Saccharolobus solfataricus
Protein Pept. Lett.
19
369-374
2012
-
-
-
-
-
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1
3
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1
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1
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1
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716542
Jung
Arabidopsis nucleoside hydrola ...
Arabidopsis thaliana
Plant J.
65
703-711
2011
-
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-
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5
1
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3
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6
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6
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2
2
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707897
Garau
Active site plasticity reveale ...
Escherichia coli
BMC Struct. Biol.
10
14
2010
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1
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1
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1
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1
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4
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1
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1
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1
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1
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2
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1
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1
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1
1
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700741
Jung
Uridine-ribohydrolase is a key ...
Arabidopsis thaliana
Plant Cell
21
876-891
2009
-
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1
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1
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6
-
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11
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1
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6
1
-
8
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-
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-
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2
-
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1
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7
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1
-
9
1
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8
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679803
Porcelli
Pyrimidine-specific ribonucleo ...
Saccharolobus solfataricus
FEBS J.
275
1900-1914
2008
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1
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2
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1
3
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6
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1
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3
1
1
1
2
2
-
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1
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1
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-
-
-
-
-
2
-
1
3
-
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-
-
1
-
-
-
-
3
1
1
1
2
2
-
-
-
1
-
-
-
-
-
-
696258
Iovane
Structural basis for substrate ...
Escherichia coli
Biochemistry
47
4418-4426
2008
-
-
1
1
10
-
-
18
-
1
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
18
-
-
-
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-
1
-
1
10
-
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18
-
1
-
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1
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2
-
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18
-
-
-
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664140
Muzzolini
New insight in to the mechanis ...
Escherichia coli K-12
Biochemistry
45
773-782
2006
-
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1
1
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-
-
6
-
-
-
3
-
1
-
-
1
1
-
-
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9
1
1
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6
1
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1
-
1
-
-
-
-
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6
-
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3
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1
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9
1
1
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6
1
-
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670166
Kim
Genes encoding ribonucleoside ...
Corynebacterium ammoniagenes
Microbiology
152
1169-1177
2006
-
-
1
-
-
-
-
-
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1
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5
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16
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5
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