Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens

Terada, M.; Tatibana, M.; Hayaishi, O.; J. Biol. Chem. 242, 5578-5585 (1967)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

adenosine

at 6.25 mM, competitive inhibitor for uridine hydrolysis

Pseudomonas fluorescens

AgCl

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

CaCl2

at 1 mM, inhibition less than 10%

Pseudomonas fluorescens

CdCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

CoCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

CuCl2

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

cytidine

at 6.25 mM, competitive inhibitor for uridine hydrolysis

Pseudomonas fluorescens

EDTA

at 1 mM: inhibits uridine hydrolysis by 50%

Pseudomonas fluorescens

FeCl3

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

FeSO4

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

HgCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

Inosine

at 6.25 mM, competitive inhibitor for uridine hydrolysis

Pseudomonas fluorescens

MgCl2

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

MnSO4

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

NH4Cl

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

ZnCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.2

-

Inosine

pH 8.5

Pseudomonas fluorescens

0.5

-

adenosine

pH 8.5

Pseudomonas fluorescens

0.5

-

Inosine

-

Pseudomonas fluorescens

0.6

-

guanosine

-

Pseudomonas fluorescens

0.8

-

uridine

-

Pseudomonas fluorescens

1

-

cytidine

pH 8.5

Pseudomonas fluorescens

2.5

-

5-Bromouridine

-

Pseudomonas fluorescens

6.5

-

Xanthosine

-

Pseudomonas fluorescens

7.5

-

1-beta-D-ribofuranosylthymine

-

Pseudomonas fluorescens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

N-D-ribosylpyrimidine

Pseudomonas fluorescens

-

pyrimidine + D-ribose

-

Pseudomonas fluorescens

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

1-beta-D-ribofuranosylnicotinamide + H2O

-

137117

Pseudomonas fluorescens

nicotinamide + D-ribose

-

137117

Pseudomonas fluorescens

?

1-beta-D-ribofuranosylthymine + H2O

-

137117

Pseudomonas fluorescens

D-ribose + thymine

-

137117

Pseudomonas fluorescens

?

4-amino-5-imidazolecarboxamide ribonucleotide + H2O

-

137117

Pseudomonas fluorescens

4-amino-5-imidazolecarboxamide + D-ribose

-

137117

Pseudomonas fluorescens

?

5-bromouridine + H2O

-

137117

Pseudomonas fluorescens

5-bromouracil + D-ribose

-

137117

Pseudomonas fluorescens

?

6-azauridine + H2O

poor substrate

137117

Pseudomonas fluorescens

6-azauracil + D-ribose

-

137117

Pseudomonas fluorescens

?

adenosine + H2O

-

137117

Pseudomonas fluorescens

adenine + D-ribose

-

137117

Pseudomonas fluorescens

?

cytidine + H2O

-

137117

Pseudomonas fluorescens

cytosine + D-ribose

-

137117

Pseudomonas fluorescens

?

guanosine + H2O

-

137117

Pseudomonas fluorescens

guanine + D-ribose

-

137117

Pseudomonas fluorescens

?

imidazoleacetic acid ribonucleotide + H2O

-

137117

Pseudomonas fluorescens

imidazoleacetic acid + D-ribose

-

137117

Pseudomonas fluorescens

?

inosine + H2O

-

137117

Pseudomonas fluorescens

hypoxanthine + D-ribose

-

137117

Pseudomonas fluorescens

?

N-D-ribosylpyrimidine

-

137117

Pseudomonas fluorescens

pyrimidine + D-ribose

-

137117

Pseudomonas fluorescens

?

N-D-ribosylpyrimidine + H2O

-

137117

Pseudomonas fluorescens

pyrimidine + D-ribose

-

137117

Pseudomonas fluorescens

?

purine D-ribonucleosides + H2O

prefers pyrimidines

137117

Pseudomonas fluorescens

purine + D-ribose

-

137117

Pseudomonas fluorescens

?

tubercidin + H2O

poor substrate

137117

Pseudomonas fluorescens

1-deazaadenine + D-ribose

-

137117

Pseudomonas fluorescens

?

uridine + H2O

-

137117

Pseudomonas fluorescens

uracil + D-ribose

-

137117

Pseudomonas fluorescens

?

xanthosine + H2O

-

137117

Pseudomonas fluorescens

xanthine + D-ribose

-

137117

Pseudomonas fluorescens

?

Inhibitors

Commentary

Organism

Structure

adenosine

at 6.25 mM, competitive inhibitor for uridine hydrolysis

Pseudomonas fluorescens

AgCl

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

CaCl2

at 1 mM, inhibition less than 10%

Pseudomonas fluorescens

CdCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

CoCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

CuCl2

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

cytidine

at 6.25 mM, competitive inhibitor for uridine hydrolysis

Pseudomonas fluorescens

EDTA

at 1 mM: inhibits uridine hydrolysis by 50%

Pseudomonas fluorescens

FeCl3

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

FeSO4

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

HgCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

Inosine

at 6.25 mM, competitive inhibitor for uridine hydrolysis

Pseudomonas fluorescens

MgCl2

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

MnSO4

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

NH4Cl

at 1 mM: inhibition less than 10%

Pseudomonas fluorescens

ZnCl2

at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%

Pseudomonas fluorescens

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.2

-

Inosine

pH 8.5

Pseudomonas fluorescens

0.5

-

adenosine

pH 8.5

Pseudomonas fluorescens

0.5

-

Inosine

-

Pseudomonas fluorescens

0.6

-

guanosine

-

Pseudomonas fluorescens

0.8

-

uridine

-

Pseudomonas fluorescens

1

-

cytidine

pH 8.5

Pseudomonas fluorescens

2.5

-

5-Bromouridine

-

Pseudomonas fluorescens

6.5

-

Xanthosine

-

Pseudomonas fluorescens

7.5

-

1-beta-D-ribofuranosylthymine

-

Pseudomonas fluorescens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

N-D-ribosylpyrimidine

Pseudomonas fluorescens

-

pyrimidine + D-ribose

-

Pseudomonas fluorescens

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

1-beta-D-ribofuranosylnicotinamide + H2O

-

137117

Pseudomonas fluorescens

nicotinamide + D-ribose

-

137117

Pseudomonas fluorescens

?

1-beta-D-ribofuranosylthymine + H2O

-

137117

Pseudomonas fluorescens

D-ribose + thymine

-

137117

Pseudomonas fluorescens

?

4-amino-5-imidazolecarboxamide ribonucleotide + H2O

-

137117

Pseudomonas fluorescens

4-amino-5-imidazolecarboxamide + D-ribose

-

137117

Pseudomonas fluorescens

?

5-bromouridine + H2O

-

137117

Pseudomonas fluorescens

5-bromouracil + D-ribose

-

137117

Pseudomonas fluorescens

?

6-azauridine + H2O

poor substrate

137117

Pseudomonas fluorescens

6-azauracil + D-ribose

-

137117

Pseudomonas fluorescens

?

adenosine + H2O

-

137117

Pseudomonas fluorescens

adenine + D-ribose

-

137117

Pseudomonas fluorescens

?

cytidine + H2O

-

137117

Pseudomonas fluorescens

cytosine + D-ribose

-

137117

Pseudomonas fluorescens

?

guanosine + H2O

-

137117

Pseudomonas fluorescens

guanine + D-ribose

-

137117

Pseudomonas fluorescens

?

imidazoleacetic acid ribonucleotide + H2O

-

137117

Pseudomonas fluorescens

imidazoleacetic acid + D-ribose

-

137117

Pseudomonas fluorescens

?

inosine + H2O

-

137117

Pseudomonas fluorescens

hypoxanthine + D-ribose

-

137117

Pseudomonas fluorescens

?

N-D-ribosylpyrimidine

-

137117

Pseudomonas fluorescens

pyrimidine + D-ribose

-

137117

Pseudomonas fluorescens

?

N-D-ribosylpyrimidine + H2O

-

137117

Pseudomonas fluorescens

pyrimidine + D-ribose

-

137117

Pseudomonas fluorescens

?

purine D-ribonucleosides + H2O

prefers pyrimidines

137117

Pseudomonas fluorescens

purine + D-ribose

-

137117

Pseudomonas fluorescens

?

tubercidin + H2O

poor substrate

137117

Pseudomonas fluorescens

1-deazaadenine + D-ribose

-

137117

Pseudomonas fluorescens

?

uridine + H2O

-

137117

Pseudomonas fluorescens

uracil + D-ribose

-

137117

Pseudomonas fluorescens

?

xanthosine + H2O

-

137117

Pseudomonas fluorescens

xanthine + D-ribose

-

137117

Pseudomonas fluorescens

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

751203

Lenz

Structural explanation for th ...

Escherichia coli

J. Comput. Aided Mol. Des.

32

1375-1388

2018

-

-

1

1

1

-

-

-

-

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-

-

-

3

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3

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1

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751391

Fan

QM/MM and MM MD Simulations o ...

Escherichia coli

J. Phys. Chem. B

122

1121-1131

2018

-

-

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1

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1

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752168

Dalberto

-

Thermodynamics, functional an ...

Leishmania braziliensis

RSC Adv.

7

48861-48875

2017

-

-

1

1

-

-

-

5

-

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1

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1

1

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5

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6

1

-

-

-

5

-

-

-

-

-

-

-

-

5

5

724342

Minici

New determinants in the cataly ...

Saccharolobus solfataricus, Saccharolobus solfataricus P2

Biochemistry

51

4590-4599

2012

-

-

1

1

-

-

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1

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2

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1

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2

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-

-

-

-

-

-

-

-

726441

Porcelli

Thermal unfolding of nucleosid ...

Saccharolobus solfataricus

Protein Pept. Lett.

19

369-374

2012

-

-

-

-

-

-

-

-

-

-

-

-

1

3

-

-

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-

-

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1

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-

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1

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1

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-

-

-

-

716542

Jung

Arabidopsis nucleoside hydrola ...

Arabidopsis thaliana

Plant J.

65

703-711

2011

-

-

-

-

-

-

-

5

1

-

-

-

-

3

-

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6

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2

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1

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6

-

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-

-

-

-

-

-

2

2

-

-

-

707897

Garau

Active site plasticity reveale ...

Escherichia coli

BMC Struct. Biol.

10

14

2010

-

-

-

1

-

-

1

-

-

1

-

1

-

4

-

-

-

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-

-

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2

-

3

1

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1

-

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1

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-

-

1

-

-

-

1

1

-

-

1

-

1

-

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-

-

-

2

-

1

-

-

-

1

-

-

-

-

1

1

-

-

-

700741

Jung

Uridine-ribohydrolase is a key ...

Arabidopsis thaliana

Plant Cell

21

876-891

2009

-

-

1

-

1

-

-

6

-

-

-

-

-

11

-

-

1

-

-

6

1

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8

-

2

-

-

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-

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-

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2

-

-

1

-

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7

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1

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9

1

-

8

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

679803

Porcelli

Pyrimidine-specific ribonucleo ...

Saccharolobus solfataricus

FEBS J.

275

1900-1914

2008

-

-

1

-

-

-

-

2

-

1

3

-

-

6

-

-

1

-

-

-

-

-

3

1

1

1

1

2

2

-

-

-

-

-

1

-

-

-

1

-

-

-

-

-

-

-

2

-

1

3

-

-

-

-

1

-

-

-

-

3

1

1

1

2

2

-

-

-

1

-

-

-

-

-

-

696258

Iovane

Structural basis for substrate ...

Escherichia coli

Biochemistry

47

4418-4426

2008

-

-

1

1

10

-

-

18

-

1

-

-

-

2

-

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1

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2

-

4

-

-

-

18

-

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-

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-

-

-

-

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1

-

1

10

-

-

-

-

18

-

1

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

18

-

-

-

-

-

-

-

-

-

-

664140

Muzzolini

New insight in to the mechanis ...

Escherichia coli K-12

Biochemistry

45

773-782

2006

-

-

1

1

-

-

-

6

-

-

-

3

-

1

-

-

1

1

-

-

-

-

9

1

4

1

-

-

6

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

6

-

-

-

3

-

-

-

1

-

-

-

-

9

1

1

-

-

6

1

-

-

-

-

-

-

-

-

-

670166

Kim

Genes encoding ribonucleoside ...

Corynebacterium ammoniagenes

Microbiology

152

1169-1177

2006

-

-

1

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

2

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

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-

-

-

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-

-

-

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-

-

-

654131

Giabbai

Cloning, purification, crystal ...

Escherichia coli

Acta Crystallogr. Sect. D

D60

524-527

2004

-

-

1

1

-

-

-

-

-

-

2

-

-

3

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

1

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

657373

Giabbai

Crystal structure to 1.7 ANG o ...

Escherichia coli

Structure

12

739-749

2004

-

-

1

1

2

-

-

8

2

-

3

-

-

4

-

-

1

1

-

-

-

-

7

1

1

-

-

-

8

-

-

-

-

-

-

-

-

-

1

-

1

2

-

-

-

-

8

2

-

3

-

-

-

-

1

-

-

-

-

7

1

-

-

-

8

-

-

-

-

-

-

-

-

-

-

663495

Giabbai

Cloning, purification, crystal ...

Escherichia coli K-12

Acta Crystallogr. Sect. D

60

524-527

2004

-

-

1

1

-

-

-

-

-

-

2

-

-

3

-

-

1

-

-

-

-

-

4

2

4

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

1

-

-

-

-

4

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

137114

Allam

-

Nature of enzymes that catalyz ...

Penicillium chrysogenum

Biochem. Syst. Ecol.

15

515-517

1987

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137115

Krenitsky

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Purine salvage enzymes in man ...

Leishmania donovani

Adv. Exp. Med. Biol.

122

51-56

1980

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7

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137116

Koszalka

Nucleosidases from Leishmania ...

Leishmania donovani

J. Biol. Chem.

254

8185-8193

1979

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7

6

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1

1

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10

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1

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4168

Murray

-

Molecular weight estimations o ...

Pisum sativum

Phytochemistry

10

2645-2648

1971

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137117

Terada

Purification and properties of ...

Pseudomonas fluorescens

J. Biol. Chem.

242

5578-5585

1967

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16

9

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16

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16

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16

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