Data extracted from this reference:
Inhibitors
adenosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
AgCl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
CaCl2
at 1 mM, inhibition less than 10%
Pseudomonas fluorescens
CdCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CoCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CuCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
cytidine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
EDTA
at 1 mM: inhibits uridine hydrolysis by 50%
Pseudomonas fluorescens
FeCl3
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
FeSO4
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
HgCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
Inosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
MgCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
MnSO4
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
NH4Cl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
ZnCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
KM Value [mM]
0.2
Inosine
pH 8.5
Pseudomonas fluorescens
0.5
adenosine
pH 8.5
Pseudomonas fluorescens
0.5
Inosine
Pseudomonas fluorescens
0.6
guanosine
Pseudomonas fluorescens
0.8
uridine
Pseudomonas fluorescens
1
cytidine
pH 8.5
Pseudomonas fluorescens
2.5
5-Bromouridine
Pseudomonas fluorescens
6.5
Xanthosine
Pseudomonas fluorescens
7.5
1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
N-D-ribosylpyrimidine
Pseudomonas fluorescens
pyrimidine + D-ribose
Pseudomonas fluorescens
?
Organism
Purification (Commentary)
partial purification, 300fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
272
at 37°C
Pseudomonas fluorescens
Storage Stability
-15°C, 1-3 mg of protein per ml, stable for 6 months
Pseudomonas fluorescens
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month
Pseudomonas fluorescens
Substrates and Products (Substrate)
1-beta-D-ribofuranosylnicotinamide + H2O
137117
Pseudomonas fluorescens
nicotinamide + D-ribose
137117
Pseudomonas fluorescens
?
1-beta-D-ribofuranosylthymine + H2O
137117
Pseudomonas fluorescens
D-ribose + thymine
137117
Pseudomonas fluorescens
?
4-amino-5-imidazolecarboxamide ribonucleotide + H2O
137117
Pseudomonas fluorescens
4-amino-5-imidazolecarboxamide + D-ribose
137117
Pseudomonas fluorescens
?
5-bromouridine + H2O
137117
Pseudomonas fluorescens
5-bromouracil + D-ribose
137117
Pseudomonas fluorescens
?
6-azauridine + H2O
poor substrate
137117
Pseudomonas fluorescens
6-azauracil + D-ribose
137117
Pseudomonas fluorescens
?
adenosine + H2O
137117
Pseudomonas fluorescens
adenine + D-ribose
137117
Pseudomonas fluorescens
?
cytidine + H2O
137117
Pseudomonas fluorescens
cytosine + D-ribose
137117
Pseudomonas fluorescens
?
guanosine + H2O
137117
Pseudomonas fluorescens
guanine + D-ribose
137117
Pseudomonas fluorescens
?
imidazoleacetic acid ribonucleotide + H2O
137117
Pseudomonas fluorescens
imidazoleacetic acid + D-ribose
137117
Pseudomonas fluorescens
?
inosine + H2O
137117
Pseudomonas fluorescens
hypoxanthine + D-ribose
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine + H2O
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
137117
Pseudomonas fluorescens
?
purine D-ribonucleosides + H2O
prefers pyrimidines
137117
Pseudomonas fluorescens
purine + D-ribose
137117
Pseudomonas fluorescens
?
tubercidin + H2O
poor substrate
137117
Pseudomonas fluorescens
1-deazaadenine + D-ribose
137117
Pseudomonas fluorescens
?
uridine + H2O
137117
Pseudomonas fluorescens
uracil + D-ribose
137117
Pseudomonas fluorescens
?
xanthosine + H2O
137117
Pseudomonas fluorescens
xanthine + D-ribose
137117
Pseudomonas fluorescens
?
Temperature Stability [°C]
45
for 10 min at pH 5.0, 50% of activity remains; for 10 min at pH 7.0-9.2, more than 90% of activity remains
Pseudomonas fluorescens
60
for 15 min, 60% denaturation
Pseudomonas fluorescens
70
for 5 min at pH 8.0, complete inactivation
Pseudomonas fluorescens
pH Optimum
6
substrate: inosine
Pseudomonas fluorescens
6.5
substrate: guanosine
Pseudomonas fluorescens
7
substrate: xanthosine
Pseudomonas fluorescens
8
substrate: 1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
8.5
substrate: cytidine, uridine, 5-bromouridine and adenosine
Pseudomonas fluorescens
Inhibitors (protein specific)
adenosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
AgCl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
CaCl2
at 1 mM, inhibition less than 10%
Pseudomonas fluorescens
CdCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CoCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
CuCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
cytidine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
EDTA
at 1 mM: inhibits uridine hydrolysis by 50%
Pseudomonas fluorescens
FeCl3
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
FeSO4
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
HgCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
Inosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
MgCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
MnSO4
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
NH4Cl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
ZnCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
KM Value [mM] (protein specific)
0.2
Inosine
pH 8.5
Pseudomonas fluorescens
0.5
adenosine
pH 8.5
Pseudomonas fluorescens
0.5
Inosine
Pseudomonas fluorescens
0.6
guanosine
Pseudomonas fluorescens
0.8
uridine
Pseudomonas fluorescens
1
cytidine
pH 8.5
Pseudomonas fluorescens
2.5
5-Bromouridine
Pseudomonas fluorescens
6.5
Xanthosine
Pseudomonas fluorescens
7.5
1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
N-D-ribosylpyrimidine
Pseudomonas fluorescens
pyrimidine + D-ribose
Pseudomonas fluorescens
?
Purification (Commentary) (protein specific)
partial purification, 300fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
272
at 37°C
Pseudomonas fluorescens
Storage Stability (protein specific)
-15°C, 1-3 mg of protein per ml, stable for 6 months
Pseudomonas fluorescens
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
1-beta-D-ribofuranosylnicotinamide + H2O
137117
Pseudomonas fluorescens
nicotinamide + D-ribose
137117
Pseudomonas fluorescens
?
1-beta-D-ribofuranosylthymine + H2O
137117
Pseudomonas fluorescens
D-ribose + thymine
137117
Pseudomonas fluorescens
?
4-amino-5-imidazolecarboxamide ribonucleotide + H2O
137117
Pseudomonas fluorescens
4-amino-5-imidazolecarboxamide + D-ribose
137117
Pseudomonas fluorescens
?
5-bromouridine + H2O
137117
Pseudomonas fluorescens
5-bromouracil + D-ribose
137117
Pseudomonas fluorescens
?
6-azauridine + H2O
poor substrate
137117
Pseudomonas fluorescens
6-azauracil + D-ribose
137117
Pseudomonas fluorescens
?
adenosine + H2O
137117
Pseudomonas fluorescens
adenine + D-ribose
137117
Pseudomonas fluorescens
?
cytidine + H2O
137117
Pseudomonas fluorescens
cytosine + D-ribose
137117
Pseudomonas fluorescens
?
guanosine + H2O
137117
Pseudomonas fluorescens
guanine + D-ribose
137117
Pseudomonas fluorescens
?
imidazoleacetic acid ribonucleotide + H2O
137117
Pseudomonas fluorescens
imidazoleacetic acid + D-ribose
137117
Pseudomonas fluorescens
?
inosine + H2O
137117
Pseudomonas fluorescens
hypoxanthine + D-ribose
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
137117
Pseudomonas fluorescens
?
N-D-ribosylpyrimidine + H2O
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
137117
Pseudomonas fluorescens
?
purine D-ribonucleosides + H2O
prefers pyrimidines
137117
Pseudomonas fluorescens
purine + D-ribose
137117
Pseudomonas fluorescens
?
tubercidin + H2O
poor substrate
137117
Pseudomonas fluorescens
1-deazaadenine + D-ribose
137117
Pseudomonas fluorescens
?
uridine + H2O
137117
Pseudomonas fluorescens
uracil + D-ribose
137117
Pseudomonas fluorescens
?
xanthosine + H2O
137117
Pseudomonas fluorescens
xanthine + D-ribose
137117
Pseudomonas fluorescens
?
Temperature Stability [°C] (protein specific)
45
for 10 min at pH 5.0, 50% of activity remains; for 10 min at pH 7.0-9.2, more than 90% of activity remains
Pseudomonas fluorescens
60
for 15 min, 60% denaturation
Pseudomonas fluorescens
70
for 5 min at pH 8.0, complete inactivation
Pseudomonas fluorescens
pH Optimum (protein specific)
6
substrate: inosine
Pseudomonas fluorescens
6.5
substrate: guanosine
Pseudomonas fluorescens
7
substrate: xanthosine
Pseudomonas fluorescens
8
substrate: 1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
8.5
substrate: cytidine, uridine, 5-bromouridine and adenosine
Pseudomonas fluorescens
Other publictions for EC
751203
Lenz
Structural explanation for th ...
Escherichia coli
J. Comput. Aided Mol. Des.
32
1375-1388
2018
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751391
Fan
QM/MM and MM MD Simulations o ...
Escherichia coli
J. Phys. Chem. B
122
1121-1131
2018
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752168
Dalberto
-
Thermodynamics, functional an ...
Leishmania braziliensis
RSC Adv.
7
48861-48875
2017
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1
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5
5
724342
Minici
New determinants in the cataly ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochemistry
51
4590-4599
2012
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726441
Porcelli
Thermal unfolding of nucleosid ...
Saccharolobus solfataricus
Protein Pept. Lett.
19
369-374
2012
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716542
Jung
Arabidopsis nucleoside hydrola ...
Arabidopsis thaliana
Plant J.
65
703-711
2011
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707897
Garau
Active site plasticity reveale ...
Escherichia coli
BMC Struct. Biol.
10
14
2010
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1
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700741
Jung
Uridine-ribohydrolase is a key ...
Arabidopsis thaliana
Plant Cell
21
876-891
2009
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6
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1
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1
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679803
Porcelli
Pyrimidine-specific ribonucleo ...
Saccharolobus solfataricus
FEBS J.
275
1900-1914
2008
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1
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1
1
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2
2
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1
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696258
Iovane
Structural basis for substrate ...
Escherichia coli
Biochemistry
47
4418-4426
2008
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1
1
10
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18
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1
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18
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10
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18
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18
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664140
Muzzolini
New insight in to the mechanis ...
Escherichia coli K-12
Biochemistry
45
773-782
2006
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1
1
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6
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1
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1
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1
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670166
Kim
Genes encoding ribonucleoside ...
Corynebacterium ammoniagenes
Microbiology
152
1169-1177
2006
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654131
Giabbai
Cloning, purification, crystal ...
Escherichia coli
Acta Crystallogr. Sect. D
D60
524-527
2004
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1
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1
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657373
Giabbai
Crystal structure to 1.7 ANG o ...
Escherichia coli
Structure
12
739-749
2004
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1
1
2
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8
2
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3
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4
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1
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1
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663495
Giabbai
Cloning, purification, crystal ...
Escherichia coli K-12
Acta Crystallogr. Sect. D
60
524-527
2004
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1
1
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2
4
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4
2
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137114
Allam
-
Nature of enzymes that catalyz ...
Penicillium chrysogenum
Biochem. Syst. Ecol.
15
515-517
1987
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1
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1
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-
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-
137115
Krenitsky
-
Purine salvage enzymes in man ...
Leishmania donovani
Adv. Exp. Med. Biol.
122
51-56
1980
-
-
-
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-
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1
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1
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7
-
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-
-
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-
137116
Koszalka
Nucleosidases from Leishmania ...
Leishmania donovani
J. Biol. Chem.
254
8185-8193
1979
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7
6
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1
1
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1
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1
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1
1
1
10
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1
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1
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7
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6
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1
1
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1
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1
1
1
10
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1
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1
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4168
Murray
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Molecular weight estimations o ...
Pisum sativum
Phytochemistry
10
2645-2648
1971
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1
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1
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3
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137117
Terada
Purification and properties of ...
Pseudomonas fluorescens
J. Biol. Chem.
242
5578-5585
1967
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16
9
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1
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2
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1
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1
2
16
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3
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5
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16
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9
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1
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1
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1
2
16
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3
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5
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