Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | at 6.25 mM, competitive inhibitor for uridine hydrolysis | Pseudomonas fluorescens | |
AgCl | at 1 mM: inhibition less than 10% | Pseudomonas fluorescens | |
CaCl2 | at 1 mM, inhibition less than 10% | Pseudomonas fluorescens | |
CdCl2 | at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% | Pseudomonas fluorescens | |
CoCl2 | at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% | Pseudomonas fluorescens | |
CuCl2 | at 1 mM: inhibition less than 10% | Pseudomonas fluorescens | |
cytidine | at 6.25 mM, competitive inhibitor for uridine hydrolysis | Pseudomonas fluorescens | |
EDTA | at 1 mM: inhibits uridine hydrolysis by 50% | Pseudomonas fluorescens | |
FeCl3 | at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% | Pseudomonas fluorescens | |
FeSO4 | at 1 mM: inhibition less than 10% | Pseudomonas fluorescens | |
HgCl2 | at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% | Pseudomonas fluorescens | |
Inosine | at 6.25 mM, competitive inhibitor for uridine hydrolysis | Pseudomonas fluorescens | |
MgCl2 | at 1 mM: inhibition less than 10% | Pseudomonas fluorescens | |
MnSO4 | at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% | Pseudomonas fluorescens | |
NH4Cl | at 1 mM: inhibition less than 10% | Pseudomonas fluorescens | |
ZnCl2 | at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Inosine | pH 8.5 | Pseudomonas fluorescens | |
0.5 | - |
Inosine | - |
Pseudomonas fluorescens | |
0.5 | - |
adenosine | pH 8.5 | Pseudomonas fluorescens | |
0.6 | - |
guanosine | - |
Pseudomonas fluorescens | |
0.8 | - |
uridine | - |
Pseudomonas fluorescens | |
1 | - |
cytidine | pH 8.5 | Pseudomonas fluorescens | |
2.5 | - |
5-Bromouridine | - |
Pseudomonas fluorescens | |
6.5 | - |
Xanthosine | - |
Pseudomonas fluorescens | |
7.5 | - |
1-beta-D-ribofuranosylthymine | - |
Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-D-ribosylpyrimidine | Pseudomonas fluorescens | - |
pyrimidine + D-ribose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification, 300fold | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
272 | - |
at 37°C | Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-15°C, 1-3 mg of protein per ml, stable for 6 months | Pseudomonas fluorescens |
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-beta-D-ribofuranosylnicotinamide + H2O | - |
Pseudomonas fluorescens | nicotinamide + D-ribose | - |
? | |
1-beta-D-ribofuranosylthymine + H2O | - |
Pseudomonas fluorescens | D-ribose + thymine | - |
? | |
4-amino-5-imidazolecarboxamide ribonucleotide + H2O | - |
Pseudomonas fluorescens | 4-amino-5-imidazolecarboxamide + D-ribose | - |
? | |
5-bromouridine + H2O | - |
Pseudomonas fluorescens | 5-bromouracil + D-ribose | - |
? | |
6-azauridine + H2O | poor substrate | Pseudomonas fluorescens | 6-azauracil + D-ribose | - |
? | |
adenosine + H2O | - |
Pseudomonas fluorescens | adenine + D-ribose | - |
? | |
cytidine + H2O | - |
Pseudomonas fluorescens | cytosine + D-ribose | - |
? | |
guanosine + H2O | - |
Pseudomonas fluorescens | guanine + D-ribose | - |
? | |
imidazoleacetic acid ribonucleotide + H2O | - |
Pseudomonas fluorescens | imidazoleacetic acid + D-ribose | - |
? | |
inosine + H2O | - |
Pseudomonas fluorescens | hypoxanthine + D-ribose | - |
? | |
N-D-ribosylpyrimidine | - |
Pseudomonas fluorescens | pyrimidine + D-ribose | - |
? | |
N-D-ribosylpyrimidine + H2O | - |
Pseudomonas fluorescens | pyrimidine + D-ribose | - |
? | |
purine D-ribonucleosides + H2O | prefers pyrimidines | Pseudomonas fluorescens | purine + D-ribose | - |
? | |
tubercidin + H2O | poor substrate | Pseudomonas fluorescens | 1-deazaadenine + D-ribose | - |
? | |
uridine + H2O | - |
Pseudomonas fluorescens | uracil + D-ribose | - |
? | |
xanthosine + H2O | - |
Pseudomonas fluorescens | xanthine + D-ribose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
for 10 min at pH 7.0-9.2, more than 90% of activity remains | Pseudomonas fluorescens |
45 | - |
for 10 min at pH 5.0, 50% of activity remains | Pseudomonas fluorescens |
60 | - |
for 15 min, 60% denaturation | Pseudomonas fluorescens |
70 | - |
for 5 min at pH 8.0, complete inactivation | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
substrate: inosine | Pseudomonas fluorescens |
6.5 | - |
substrate: guanosine | Pseudomonas fluorescens |
7 | - |
substrate: xanthosine | Pseudomonas fluorescens |
8 | - |
substrate: 1-beta-D-ribofuranosylthymine | Pseudomonas fluorescens |
8.5 | - |
substrate: cytidine, uridine, 5-bromouridine and adenosine | Pseudomonas fluorescens |