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Literature summary for 3.2.2.7 extracted from

  • Thicklin, L.; Shamsuddin, A.; Alahmry, F.; Gezley, C.; Brown, E.; Stone, J.; Burns-Carver, E.; Kline, P.C.
    Purification of a non-specific nucleoside hydrolase from Alaska pea seeds (2019), Protein Expr. Purif., 154, 140-146 .
    View publication on PubMed
Search for more literature for 3.2.2.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.184
-
 adenosine pH 7.2, 32°C Pisum sativum
0.283
-
 Inosine pH 7.2, 32°C Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from seed Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.308
-
 pH 7.2, 32°C Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-5-methyluridine + H2O
-
 Pisum sativum 5-methyluracil + 2-deoxy-D-ribose
-
 ?
2'-deoxyadenosine + H2O
-
 Pisum sativum 6-aminopurine + 2-deoxy-D-ribose
-
 ?
2'-deoxycytidine + H2O
-
 Pisum sativum cytosine + 2-deoxy-D-ribose
-
 ?
2'-deoxyguanosine + H2O
-
 Pisum sativum guanine + 2-deoxy-D-ribose
-
 ?
2'-deoxyinosine + H2O
-
 Pisum sativum hypoxanthine + 2-deoxy-D-ribose
-
 ?
2'-deoxyuridine + H2O
-
 Pisum sativum uracil + 2-deoxy-D-ribose
-
 ?
5-methyluridine + H2O
-
 Pisum sativum 5-methyluracil + D-ribose
-
 ?
adenosine + H2O best substrate Pisum sativum adenine + D-ribose
-
 ?
cytidine + H2O
-
 Pisum sativum cytosine + D-ribose
-
 ?
guanosine + H2O
-
 Pisum sativum guanine + D-ribose
-
 ?
inosine + H2O
-
 Pisum sativum hypoxanthine + D-ribose
-
 ?
additional information all of the ribonucleosides tested are better substrates than their corresponding 2'-deoxynucleosides. The purines are better substrates than the pyrimidines Pisum sativum ?
-
 ?
uridine + H2O
-
 Pisum sativum uracil + D-ribose
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE, x * 26103, mass spectrometry Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pisum sativum