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Literature summary for 3.2.2.7 extracted from

  • Guranowski, A.; Schneider, Z.
    Purification and characterization of adenosine nucleosidase from barley leaves (1977), Biochim. Biophys. Acta, 482, 145-158.
    View publication on PubMed
Search for more literature for 3.2.2.7

General Stability

General Stability Organism
presence of salt essential Hordeum vulgare
stable against repeated freezing and thawing cycles Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyadenosine Ki: 0.05 mM Hordeum vulgare
3'-deoxyadenosine Ki: four times weaker than for 2'-deoxyadenosine Hordeum vulgare
adenine strong competitive inhibitor Hordeum vulgare
cytokinin plant hormones, inhibition less strong than for adenine Hordeum vulgare
dithiothreitol 60% inhibition if enzyme is preincubated with 10 mM Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 adenosine in MES buffer, pH 5.4 Hordeum vulgare
0.12
-
 2'-deoxyadenosine in MES buffer, pH 5.4 Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 alpha2, 2 * 33000, non-reducing SDS-PAGE Hordeum vulgare
66000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxyadenosine + H2O Hordeum vulgare 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
adenosine + H2O Hordeum vulgare 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 barley
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Hordeum vulgare

Reaction

Reaction Comment Organism Reaction ID
adenosine + H2O = D-ribose + adenine also acts on adenosine N-oxide Hordeum vulgare
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.5
-
-
 Hordeum vulgare

Storage Stability

Storage Stability Organism
storage in dried state in Sephadex G-25 beads remains stability for several months Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Hordeum vulgare 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
6-aminopurine-9-beta-D-ribofuranosyl-N1-oxide + H2O same hydrolysis rate than for adenosine or 2'-deoxyadenosine Hordeum vulgare 6-aminopurine-N1-oxide + D-ribofuranose
-
 ?
adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Hordeum vulgare 6-aminopurine + D-ribofuranose
-
 ?
purine riboside + H2O low activity Hordeum vulgare purine + D-ribose
-
 ?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 33000, non-reducing SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
-
 Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 in 40 mM citrate buffer Hordeum vulgare
5.4
-
 in 50 mM MES/KOH buffer Hordeum vulgare

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
 2'-deoxyadenosine
-
 Hordeum vulgare