Cloned (Comment) | Organism |
---|---|
gene cd38, eight exons, DNA and amino acid sequence determination and analysis, sequence comparison to the human enzyme, phylogenetic tree, expression in COS-7 cells | Xenopus laevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | most frog enzyme (81%) is distributed in the membrane fraction (5% in mitochondrial fraction and 14% in microsomal fraction) | Xenopus laevis | 16020 | - |
microsome | - |
Xenopus laevis | - |
- |
mitochondrion | mitochondrial membrane | Xenopus laevis | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28970 | - |
x * 45000, SDS-PAGE, x * 28970, sequence calculation | Xenopus laevis |
45000 | - |
x * 45000, SDS-PAGE, x * 28970, sequence calculation | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclic ADP-ribose + H2O | Xenopus laevis | - |
ADP-D-ribose | - |
? | |
additional information | Xenopus laevis | frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities | ? | - |
? | |
NAD+ | Xenopus laevis | - |
cyclic ADP-ribose + nicotinamide | - |
? | |
NAD+ + H2O | Xenopus laevis | overall reaction | ADP-D-ribose + nicotinamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | H7C831 | gene cd38 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Xenopus laevis | - |
liver | - |
Xenopus laevis | - |
additional information | the enzyme is ubiquitously expressed in various tissues | Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0604 | - |
NAD+-glycohydrolase activityin crude cell extract, pH 7.4, 37°C | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclic ADP-ribose + H2O | - |
Xenopus laevis | ADP-D-ribose | - |
? | |
additional information | frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities | Xenopus laevis | ? | - |
? | |
NAD+ | - |
Xenopus laevis | cyclic ADP-ribose + nicotinamide | - |
? | |
NAD+ + H2O | overall reaction | Xenopus laevis | ADP-D-ribose + nicotinamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE, x * 28970, sequence calculation | Xenopus laevis |
Synonyms | Comment | Organism |
---|---|---|
CD38 | - |
Xenopus laevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Xenopus laevis |
General Information | Comment | Organism |
---|---|---|
evolution | the CD38-cADPR signaling system is conserved during vertebrate evolution, phylogenetic tree | Xenopus laevis |
physiological function | the enzyme is CD38-cADPR signaling system in frog cells | Xenopus laevis |