BRENDA - Reference to 3.2.2.6; Id = 326409

50% inactivation at 2 M urea

Homo sapiens

SDS, even at micromolar concentration, leads to complete inactivation of the enzyme

Homo sapiens

Ag+

50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity

Homo sapiens

Cr3+

; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

Hg2+

complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

Pb2+

complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

SDS

-

Homo sapiens

Sn2+

complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

Zn2+

; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

0.026

-

NAD+

; pH 7.0, 28°C

Homo sapiens

0.033

-

epsilonNAD+

-

Homo sapiens

0.033

-

nicotinamide 1,N6-ethenoadenine dinucleotide

pH 7.0, 28°C

Homo sapiens

membrane

-

Homo sapiens

16020

-

microsome

-

Homo sapiens

-

Ag+

50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity

Homo sapiens

Cu2+

stimulation of cADPR activity

Homo sapiens

additional information

no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+

Homo sapiens

additional information

-

native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column

Homo sapiens

45000

-

Homo sapiens

additional information

Homo sapiens

enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose

?

-

Homo sapiens

-

partial; partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold

Homo sapiens

NAD+ + H2O = ADP-D-ribose + nicotinamide

also catalyses transfer of ADP-ribose(P) residues

Homo sapiens

80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS

Homo sapiens

spleen

-

Homo sapiens

-

0.02

-

partially purified enzyme, ADPR activity

Homo sapiens

0.113

-

partially purified enzyme, cADPR activity

Homo sapiens

2.205

-

partially purified enzyme, NADase activity

Homo sapiens

20°C, lyophilized enzyme, 90% remaining activity after 6 months

Homo sapiens

4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable

Homo sapiens

4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month

Homo sapiens

3-acetylpyridine + adenosine diphosphoribose

transglycosylation activity, very poor hydrolytic activity with this substrate

326409

Homo sapiens

3-acetylpyridine adenine dinucleotide + phosphate

-

?

3-acetylpyridine adenine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

3-acetylpyridine hypoxanthine dinucleotide + H2O

low activity

326409

Homo sapiens

?

-

?

3-pyridine adenine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

3-pyridinealdehyde hypoxanthine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

3-pyridinealdehyde hypoxanthine dinucleotide + H2O

60% activity compared to the activity with NAD+

326409

Homo sapiens

?

-

?

cyclic ADP-ribose + H2O

-

326409

Homo sapiens

?

-

?

cyclic ADP-ribose + H2O

i.e. ADPR activity

326409

Homo sapiens

adenosine diphosphoribose + ?

-

?

epsilon-NAD+ + H2O

-

326409

Homo sapiens

?

-

?

epsilon-NADP+ + H2O

-

326409

Homo sapiens

?

-

?

additional information

enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose

326409

Homo sapiens

?

-

additional information

enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose

326409

Homo sapiens

?

-

NAD+

-

326409

Homo sapiens

cyclic ADP-ribose + nicotinamide

-

326409

Homo sapiens

?

NAD+ + ?

i.e. cADPR activity

326409

Homo sapiens

cyclic ADP-ribose + ?

-

?

NAD+ + H2O

-

326409

Homo sapiens

ADP-ribose + nicotinamide

-

?

NADP+ + H2O

-

326409

Homo sapiens

nicotinamide + ADPribose-phosphate

-

?

NADP+ + H2O

80% activity compared to the activity with NAD+

326409

Homo sapiens

ADP-ribose-P + nicotinamide

-

?

NGD+ + H2O

as effective as substrate as NAD+

326409

Homo sapiens

GDP-ribose + nicotinamide

-

?

nicotinamide 1,N6-ethenoadenine dinucleotide + H2O

i.e. epsilonNAD+, used for a fluorometric assay

326409

Homo sapiens

?

-

?

nicotinamide guanine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

nicotinamide-hypoxanthine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

thio-NAD+ + H2O

-

326409

Homo sapiens

?

-

?

oligomer

x * 45000, SDS-PAGE

Homo sapiens

38

-

Homo sapiens

38

50

inactive above 50°C

Homo sapiens

38

-

half-life: about 1 day

Homo sapiens

7

-

; NADase activity

Homo sapiens

4.5

9.5

-

Homo sapiens

isoelectric focusing

-

4.9

50% inactivation at 2 M urea

Homo sapiens

SDS, even at micromolar concentration, leads to complete inactivation of the enzyme

Homo sapiens

Ag+

50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity

Homo sapiens

Cr3+

; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

Hg2+

complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

Pb2+

complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

SDS

-

Homo sapiens

Sn2+

complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

Zn2+

; inhibition of NADase and adenosine diphosphate cyclase

Homo sapiens

0.026

-

NAD+

; pH 7.0, 28°C

Homo sapiens

0.033

-

epsilonNAD+

-

Homo sapiens

0.033

-

nicotinamide 1,N6-ethenoadenine dinucleotide

pH 7.0, 28°C

Homo sapiens

membrane

-

Homo sapiens

16020

-

microsome

-

Homo sapiens

-

Ag+

50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity

Homo sapiens

Cu2+

stimulation of cADPR activity

Homo sapiens

additional information

no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+

Homo sapiens

additional information

-

native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column

Homo sapiens

45000

-

Homo sapiens

additional information

Homo sapiens

enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose

?

-

partial; partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold

Homo sapiens

80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS

Homo sapiens

spleen

-

Homo sapiens

-

0.02

-

partially purified enzyme, ADPR activity

Homo sapiens

0.113

-

partially purified enzyme, cADPR activity

Homo sapiens

2.205

-

partially purified enzyme, NADase activity

Homo sapiens

20°C, lyophilized enzyme, 90% remaining activity after 6 months

Homo sapiens

4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable

Homo sapiens

4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month

Homo sapiens

3-acetylpyridine + adenosine diphosphoribose

transglycosylation activity, very poor hydrolytic activity with this substrate

326409

Homo sapiens

3-acetylpyridine adenine dinucleotide + phosphate

-

?

3-acetylpyridine adenine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

3-acetylpyridine hypoxanthine dinucleotide + H2O

low activity

326409

Homo sapiens

?

-

?

3-pyridine adenine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

3-pyridinealdehyde hypoxanthine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

3-pyridinealdehyde hypoxanthine dinucleotide + H2O

60% activity compared to the activity with NAD+

326409

Homo sapiens

?

-

?

cyclic ADP-ribose + H2O

-

326409

Homo sapiens

?

-

?

cyclic ADP-ribose + H2O

i.e. ADPR activity

326409

Homo sapiens

adenosine diphosphoribose + ?

-

?

epsilon-NAD+ + H2O

-

326409

Homo sapiens

?

-

?

epsilon-NADP+ + H2O

-

326409

Homo sapiens

?

-

?

additional information

enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose

326409

Homo sapiens

?

-

additional information

enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose

326409

Homo sapiens

?

-

NAD+

-

326409

Homo sapiens

cyclic ADP-ribose + nicotinamide

-

326409

Homo sapiens

?

NAD+ + ?

i.e. cADPR activity

326409

Homo sapiens

cyclic ADP-ribose + ?

-

?

NAD+ + H2O

-

326409

Homo sapiens

ADP-ribose + nicotinamide

-

?

NADP+ + H2O

-

326409

Homo sapiens

nicotinamide + ADPribose-phosphate

-

?

NADP+ + H2O

80% activity compared to the activity with NAD+

326409

Homo sapiens

ADP-ribose-P + nicotinamide

-

?

NGD+ + H2O

as effective as substrate as NAD+

326409

Homo sapiens

GDP-ribose + nicotinamide

-

?

nicotinamide 1,N6-ethenoadenine dinucleotide + H2O

i.e. epsilonNAD+, used for a fluorometric assay

326409

Homo sapiens

?

-

?

nicotinamide guanine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

nicotinamide-hypoxanthine dinucleotide + H2O

-

326409

Homo sapiens

?

-

?

thio-NAD+ + H2O

-

326409

Homo sapiens

?

-

?

oligomer

x * 45000, SDS-PAGE

Homo sapiens

38

-

Homo sapiens

38

50

inactive above 50°C

Homo sapiens

38

-

half-life: about 1 day

Homo sapiens

7

-

; NADase activity

Homo sapiens

4.5

9.5

-

Homo sapiens

isoelectric focusing

-

4.9

749830

Chini

The NADase CD38 is induced by ...

Homo sapiens

Biochem. Biophys. Res. Commun.

513

486-493

2019

-

1

-

1

-

1

-

1

-

750282

Shu

Blockade of CD38 diminishes l ...

Mus musculus

Cell. Signal.

42

249-258

2018

-

4

-

1

-

1

-

1

-

731357

Kuhn

Probing the catalytic mechanis ...

Bos taurus

Biochim. Biophys. Acta

1844

1317-1331

2014

-

11

-

7

12

-

1

-

2

-

1

-

3

-

1

-

12

1

-

11

-

7

-

12

-

1

-

3

-

1

-

12

1

-

1

-

12

731882

Ma

Basal CD38/cyclic ADP-ribose-d ...

Mus musculus

Glia

62

943-955

2014

-

4

-

1

-

4

-

4

-

1

-

1

-

4

-

4

-

4

-

1

-

1

-

2

-

731987

Shrimp

Revealing CD38 cellular locali ...

Homo sapiens

J. Am. Chem. Soc.

136

5656-5663

2014

-

2

-

2

-

3

-

1

-

2

-

3

-

1

-

1

-

2

-

2

-

3

-

2

-

3

-

1

-

1

-

1

-

731119

Jiang

Identification of ADP-ribosyla ...

Homo sapiens

Anal. Biochem.

433

218-226

2013

-

6

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

6

-

1

-

1

-

1

-

1

-

1

-

1

-

732593

Coskun

Purification of NAD+ glycohydr ...

Homo sapiens

Oncol. Lett.

6

227-231

2013

-

2

-

2

3

-

1

-

1

-

1

-

3

1

-

1

-

2

-

2

3

-

1

-

1

-

3

1

-

1

-

731296

Kwong

Catalysis-based inhibitors of ...

Homo sapiens, Mus musculus, Rattus norvegicus

Biochemistry

51

555-564

2012

-

1

-

42

-

9

-

3

-

4

-

9

-

3

-

3

-

1

-

42

-

9

-

4

-

9

-

3

-

3

-

9

-

732501

Ikeda

Identification of a major enzy ...

Xenopus laevis

Mol. Cell. Biochem.

366

69-80

2012

-

1

-

3

-

2

4

-

3

-

1

-

3

1

-

4

1

-

1

-

1

-

3

-

2

4

-

1

-

3

1

-

4

1

-

1

-

2

-

732691

Egea

Insights into the mechanism of ...

Bos taurus

PLoS ONE

7

e34918

2012

-

1

6

-

7

-

2

-

2

-

1

-

1

-

2

-

1

-

1

-

1

-

1

6

-

7

-

2

-

1

-

2

-

1

-

1

-

1

-

714434

Kellenberger

Flavonoids as inhibitors of hu ...

Homo sapiens

Bioorg. Med. Chem. Lett.

21

3939-3942

2011

-

1

-

17

-

1

-

1

-

2

-

16

-

1

-

16

17

-

1

-

2

-

714921

Congleton

ATRA-induced HL-60 myeloid leu ...

Homo sapiens

Exp. Cell Res.

317

910-919

2011

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

716910

Lee

Cyclic ADP-ribose and NAADP: f ...

Mus musculus

Sci. China Life Sci.

54

699-711

2011

-

1

-

3

-

1

-

3

-

1

-

1

-

3

-

1

-

326409

Orsomando

NAD(P)+-glycohydrolase from hu ...

Homo sapiens

Comp. Biochem. Physiol. B

126

89-98

2000

-

2

7

3

2

3

2

1

-

3

-

1

3

22

1

-

1

-

1

-

2

-

7

-

3

2

3

2

1

-

1

3

22

1

-

1

-

1

-

326410

Berthelier

-

Human CD38 is an authentic NAD ...

Homo sapiens

Biochem. J.

330

1383-1390

1998

-

1

6

-

1

-

1

-

9

-

1

-

6

-

1

-

9

-

326411

Chini

Enzymatic synthesis and degrad ...

Rattus norvegicus

Biochem. Biophys. Res. Commun.

209

167-174

1995

-

2

-

4

-

1

-

4

-

1

-

326406

Jorge

Carbon source regulation of ni ...

Neurospora crassa

J. Gen. Microbiol.

130

1563-1568

1984

-

1

-

2

-

3

-

2

-

1

-

3

-

2

-

326403

Yamasaki

-

Purification and some properti ...

Saccharomyces cerevisiae

J. Ferment. Technol.

60

131-137

1982

-

2

1

-

2

-

1

-

1

-

2

-

2

-

1

-

2

-

1

-

1

-

2

-

1

-

2

-

1

-

2

-

2

-

1

-

2

-

1

-

1

-

326407

Skala

Elevated NAD(P) glycohydrolase ...

Homo sapiens

Blood

60

912-917

1982

-

1

-

1

-

1

-

326408

Artman

Nicotinamide adenine dinucleot ...

Oryctolagus cuniculus, Ovis aries

Can. J. Microbiol.

28

696-702

1982

-

2

-

2

-

1

-

2

-

1

-

326405

Broeker

-

A nicotinamide adenine dinucle ...

Streptomyces griseus

FEMS Microbiol. Lett.

6

245-247

1979

-

1

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

326404

Mather

A heat-stable nicotinamide-ade ...

Pseudomonas putida KB1

Biochem. J.

129

141-152

1972

-

1

5

-

1

-

1

-

1

-

2

5

-

1

-

1

-

2

-

2

-

1

-

5

-

1

-

1

-

2

5

-

1

-

1

-

2

-

2

-

NAD(P)+-glycohydrolase from human spleen: a multicatalytic enzyme

Data extracted from this reference: