Data extracted from this reference:
General Stability
50% inactivation at 2 M urea
Homo sapiens
SDS, even at micromolar concentration, leads to complete inactivation of the enzyme
Homo sapiens
Inhibitors
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity
Homo sapiens
Cr3+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Hg2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Pb2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Sn2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Zn2+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
KM Value [mM]
0.026
NAD+
; pH 7.0, 28°C
Homo sapiens
0.033
epsilonNAD+
Homo sapiens
0.033
nicotinamide 1,N6-ethenoadenine dinucleotide
pH 7.0, 28°C
Homo sapiens
Localization
membrane
Homo sapiens
16020
Metals/Ions
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity
Homo sapiens
Cu2+
stimulation of cADPR activity
Homo sapiens
additional information
no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+
Homo sapiens
Molecular Weight [Da]
additional information
native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column
Homo sapiens
Natural Substrates/ Products (Substrates)
additional information
Homo sapiens
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
?
Organism
Purification (Commentary)
partial; partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold
Homo sapiens
Reaction
NAD+ + H2O = ADP-D-ribose + nicotinamide
also catalyses transfer of ADP-ribose(P) residues
Homo sapiens
Renatured (Commentary)
80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS
Homo sapiens
Source Tissue
Specific Activity [micromol/min/mg]
0.02
partially purified enzyme, ADPR activity
Homo sapiens
0.113
partially purified enzyme, cADPR activity
Homo sapiens
2.205
partially purified enzyme, NADase activity
Homo sapiens
Storage Stability
20°C, lyophilized enzyme, 90% remaining activity after 6 months
Homo sapiens
4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable
Homo sapiens
4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month
Homo sapiens
Substrates and Products (Substrate)
3-acetylpyridine + adenosine diphosphoribose
transglycosylation activity, very poor hydrolytic activity with this substrate
326409
Homo sapiens
3-acetylpyridine adenine dinucleotide + phosphate
?
3-acetylpyridine adenine dinucleotide + H2O
326409
Homo sapiens
?
?
3-acetylpyridine hypoxanthine dinucleotide + H2O
low activity
326409
Homo sapiens
?
?
3-pyridine adenine dinucleotide + H2O
326409
Homo sapiens
?
?
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
326409
Homo sapiens
?
?
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
60% activity compared to the activity with NAD+
326409
Homo sapiens
?
?
cyclic ADP-ribose + H2O
326409
Homo sapiens
?
?
cyclic ADP-ribose + H2O
i.e. ADPR activity
326409
Homo sapiens
adenosine diphosphoribose + ?
?
epsilon-NAD+ + H2O
326409
Homo sapiens
?
?
epsilon-NADP+ + H2O
326409
Homo sapiens
?
?
additional information
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
326409
Homo sapiens
?
additional information
enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose
326409
Homo sapiens
?
NAD+
326409
Homo sapiens
cyclic ADP-ribose + nicotinamide
326409
Homo sapiens
?
NAD+ + ?
i.e. cADPR activity
326409
Homo sapiens
cyclic ADP-ribose + ?
?
NAD+ + H2O
326409
Homo sapiens
ADP-ribose + nicotinamide
?
NADP+ + H2O
326409
Homo sapiens
nicotinamide + ADPribose-phosphate
?
NADP+ + H2O
80% activity compared to the activity with NAD+
326409
Homo sapiens
ADP-ribose-P + nicotinamide
?
NGD+ + H2O
as effective as substrate as NAD+
326409
Homo sapiens
GDP-ribose + nicotinamide
?
nicotinamide 1,N6-ethenoadenine dinucleotide + H2O
i.e. epsilonNAD+, used for a fluorometric assay
326409
Homo sapiens
?
?
nicotinamide guanine dinucleotide + H2O
326409
Homo sapiens
?
?
nicotinamide-hypoxanthine dinucleotide + H2O
326409
Homo sapiens
?
?
thio-NAD+ + H2O
326409
Homo sapiens
?
?
Subunits
oligomer
x * 45000, SDS-PAGE
Homo sapiens
Synonyms
NAD(P)+-glycohydrolase
Homo sapiens
Temperature Optimum [°C]
Temperature Range [°C]
38
50
inactive above 50°C
Homo sapiens
Temperature Stability [°C]
38
half-life: about 1 day
Homo sapiens
pH Optimum
7
; NADase activity
Homo sapiens
pH Range
pI Value
Homo sapiens
isoelectric focusing
4.9
General Stability (protein specific)
50% inactivation at 2 M urea
Homo sapiens
SDS, even at micromolar concentration, leads to complete inactivation of the enzyme
Homo sapiens
Inhibitors (protein specific)
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity
Homo sapiens
Cr3+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Hg2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Pb2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Sn2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
Zn2+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
KM Value [mM] (protein specific)
0.026
NAD+
; pH 7.0, 28°C
Homo sapiens
0.033
epsilonNAD+
Homo sapiens
0.033
nicotinamide 1,N6-ethenoadenine dinucleotide
pH 7.0, 28°C
Homo sapiens
Localization (protein specific)
membrane
Homo sapiens
16020
Metals/Ions (protein specific)
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity
Homo sapiens
Cu2+
stimulation of cADPR activity
Homo sapiens
additional information
no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+
Homo sapiens
Molecular Weight [Da] (protein specific)
additional information
native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
additional information
Homo sapiens
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
?
Purification (Commentary) (protein specific)
partial; partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold
Homo sapiens
Renatured (Commentary) (protein specific)
80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS
Homo sapiens
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
0.02
partially purified enzyme, ADPR activity
Homo sapiens
0.113
partially purified enzyme, cADPR activity
Homo sapiens
2.205
partially purified enzyme, NADase activity
Homo sapiens
Storage Stability (protein specific)
20°C, lyophilized enzyme, 90% remaining activity after 6 months
Homo sapiens
4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable
Homo sapiens
4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month
Homo sapiens
Substrates and Products (Substrate) (protein specific)
3-acetylpyridine + adenosine diphosphoribose
transglycosylation activity, very poor hydrolytic activity with this substrate
326409
Homo sapiens
3-acetylpyridine adenine dinucleotide + phosphate
?
3-acetylpyridine adenine dinucleotide + H2O
326409
Homo sapiens
?
?
3-acetylpyridine hypoxanthine dinucleotide + H2O
low activity
326409
Homo sapiens
?
?
3-pyridine adenine dinucleotide + H2O
326409
Homo sapiens
?
?
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
326409
Homo sapiens
?
?
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
60% activity compared to the activity with NAD+
326409
Homo sapiens
?
?
cyclic ADP-ribose + H2O
326409
Homo sapiens
?
?
cyclic ADP-ribose + H2O
i.e. ADPR activity
326409
Homo sapiens
adenosine diphosphoribose + ?
?
epsilon-NAD+ + H2O
326409
Homo sapiens
?
?
epsilon-NADP+ + H2O
326409
Homo sapiens
?
?
additional information
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
326409
Homo sapiens
?
additional information
enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose
326409
Homo sapiens
?
NAD+
326409
Homo sapiens
cyclic ADP-ribose + nicotinamide
326409
Homo sapiens
?
NAD+ + ?
i.e. cADPR activity
326409
Homo sapiens
cyclic ADP-ribose + ?
?
NAD+ + H2O
326409
Homo sapiens
ADP-ribose + nicotinamide
?
NADP+ + H2O
326409
Homo sapiens
nicotinamide + ADPribose-phosphate
?
NADP+ + H2O
80% activity compared to the activity with NAD+
326409
Homo sapiens
ADP-ribose-P + nicotinamide
?
NGD+ + H2O
as effective as substrate as NAD+
326409
Homo sapiens
GDP-ribose + nicotinamide
?
nicotinamide 1,N6-ethenoadenine dinucleotide + H2O
i.e. epsilonNAD+, used for a fluorometric assay
326409
Homo sapiens
?
?
nicotinamide guanine dinucleotide + H2O
326409
Homo sapiens
?
?
nicotinamide-hypoxanthine dinucleotide + H2O
326409
Homo sapiens
?
?
thio-NAD+ + H2O
326409
Homo sapiens
?
?
Subunits (protein specific)
oligomer
x * 45000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
38
50
inactive above 50°C
Homo sapiens
Temperature Stability [°C] (protein specific)
38
half-life: about 1 day
Homo sapiens
pH Optimum (protein specific)
7
; NADase activity
Homo sapiens
pH Range (protein specific)
pI Value (protein specific)
Homo sapiens
isoelectric focusing
4.9
Other publictions for EC
749830
Chini
The NADase CD38 is induced by ...
Homo sapiens
Biochem. Biophys. Res. Commun.
513
486-493
2019
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
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1
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-
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
750282
Shu
Blockade of CD38 diminishes l ...
Mus musculus
Cell. Signal.
42
249-258
2018
-
-
-
-
-
-
-
-
-
-
-
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-
4
-
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1
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1
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1
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-
-
-
-
-
-
-
1
1
1
1
-
-
731357
Kuhn
Probing the catalytic mechanis ...
Bos taurus
Biochim. Biophys. Acta
1844
1317-1331
2014
-
-
-
-
11
-
7
12
-
-
-
1
-
2
-
-
-
1
-
-
-
-
3
-
2
1
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
11
-
-
7
-
12
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
12
1
-
-
-
-
1
1
-
12
12
731882
Ma
Basal CD38/cyclic ADP-ribose-d ...
Mus musculus
Glia
62
943-955
2014
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
4
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
4
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
731987
Shrimp
Revealing CD38 cellular locali ...
Homo sapiens
J. Am. Chem. Soc.
136
5656-5663
2014
-
-
-
-
2
-
-
-
2
-
-
3
-
1
-
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-
2
-
-
3
-
1
1
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-
-
1
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-
-
-
2
-
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-
-
-
2
-
-
3
-
-
-
-
-
2
-
-
3
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
731119
Jiang
Identification of ADP-ribosyla ...
Homo sapiens
Anal. Biochem.
433
218-226
2013
-
-
-
-
6
-
-
-
-
-
-
1
-
1
-
1
-
1
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
732593
Coskun
Purification of NAD+ glycohydr ...
Homo sapiens
Oncol. Lett.
6
227-231
2013
-
-
-
-
-
-
-
-
2
-
2
3
-
1
-
-
1
-
-
1
-
-
3
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
3
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
731296
Kwong
Catalysis-based inhibitors of ...
Homo sapiens, Mus musculus, Rattus norvegicus
Biochemistry
51
555-564
2012
-
-
-
1
-
-
42
-
-
-
-
9
-
3
-
-
-
-
-
4
-
-
9
-
3
3
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
42
-
-
-
-
-
9
-
-
-
-
-
4
-
-
9
-
3
-
-
-
3
-
-
-
-
9
9
-
-
-
732501
Ikeda
Identification of a major enzy ...
Xenopus laevis
Mol. Cell. Biochem.
366
69-80
2012
-
-
1
-
-
-
-
-
3
-
2
4
-
3
-
1
-
-
-
3
1
-
4
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
2
4
-
-
1
-
-
3
1
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
732691
Egea
Insights into the mechanism of ...
Bos taurus
PLoS ONE
7
e34918
2012
-
-
1
1
6
-
-
7
-
-
-
2
-
2
-
1
-
1
-
-
-
-
2
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
6
-
-
-
-
7
-
-
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
714434
Kellenberger
Flavonoids as inhibitors of hu ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
3939-3942
2011
-
-
1
-
-
-
17
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
16
-
-
1
-
-
-
-
16
17
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714921
Congleton
ATRA-induced HL-60 myeloid leu ...
Homo sapiens
Exp. Cell Res.
317
910-919
2011
-
-
-
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
716910
Lee
Cyclic ADP-ribose and NAADP: f ...
Mus musculus
Sci. China Life Sci.
54
699-711
2011
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
326409
Orsomando
NAD(P)+-glycohydrolase from hu ...
Homo sapiens
Comp. Biochem. Physiol. B
126
89-98
2000
-
-
-
-
-
2
7
3
3
3
2
1
-
3
-
-
1
1
1
2
3
3
22
1
2
1
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
2
-
7
-
3
3
3
2
1
-
-
-
1
1
2
3
3
22
1
1
1
1
-
1
1
-
1
-
-
-
-
-
-
326410
Berthelier
-
Human CD38 is an authentic NAD ...
Homo sapiens
Biochem. J.
330
1383-1390
1998
-
-
-
-
-
-
1
6
-
-
-
-
-
1
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
326411
Chini
Enzymatic synthesis and degrad ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
209
167-174
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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4
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Jorge
Carbon source regulation of ni ...
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1984
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326403
Yamasaki
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Purification and some properti ...
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131-137
1982
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326407
Skala
Elevated NAD(P) glycohydrolase ...
Homo sapiens
Blood
60
912-917
1982
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326408
Artman
Nicotinamide adenine dinucleot ...
Oryctolagus cuniculus, Ovis aries
Can. J. Microbiol.
28
696-702
1982
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326405
Broeker
-
A nicotinamide adenine dinucle ...
Streptomyces griseus
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6
245-247
1979
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326404
Mather
A heat-stable nicotinamide-ade ...
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1972
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