Cloned (Comment) | Organism |
---|---|
co-expression of N-terminally His6-tagged enzyme with inhibitor IFS in Escherichia coli strain Rosetta2 (DE3) | Streptococcus pyogenes serotype M3 |
Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionine-labeled enzyme C-terminal domain (residues 193-451) in complex with the full-length inhibitor IFS, sitting drop vapor-diffusion method, mixing of 0.001 ml of protein solution with 0.001 ml of reservoir solution of 20% w/v tacsimate, pH 4.0, and 20% w/v PEG 3350, 22°C, X-ray diffraction structure determination and analysis at 1.70 A resolution, single-wavelength anomalous diffraction, modelling | Streptococcus pyogenes serotype M3 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Streptococcal NAD glycohydrolase inhibitor | the ifs gene, or SpyM3_0129, encodes an endogenous competitive inhibitor for the enzyme. IFS is localized intracellularly and forms a complex with the enzyme. This intracellular complex must be dissociated during export through the cell envelope. The interface between SPNct and IFS is highly rich in water molecules, binding structure, overview | Streptococcus pyogenes serotype M3 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme is injected across the host cell membrane into the cytoplasm through streptolysin O, a member of a large family of pore-forming toxins and cholesterol-dependent cytolysins | Streptococcus pyogenes serotype M3 | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, recombinant His6-tagged enzyme, SDS-PAGE | Streptococcus pyogenes serotype M3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + H2O | Streptococcus pyogenes serotype M3 | - |
ADP-D-ribose + nicotinamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes serotype M3 | A0A0H2UTN5 | gene spn or SpyM3_0128 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration | Streptococcus pyogenes serotype M3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | lack of ADP-ribosyltransferase activity of the enzyme | Streptococcus pyogenes serotype M3 | ? | - |
? | |
NAD+ + H2O | - |
Streptococcus pyogenes serotype M3 | ADP-D-ribose + nicotinamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, recombinant His6-tagged enzyme, SDS-PAGE | Streptococcus pyogenes serotype M3 |
Synonyms | Comment | Organism |
---|---|---|
beta-NAD+glycohydrolase | - |
Streptococcus pyogenes serotype M3 |
SPN | - |
Streptococcus pyogenes serotype M3 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is one of the virulence factors produced by Streptococcus pyogenes, which injects the enzyme into the cytosol of an infected host cell using the cytolysin-mediated translocation pathway. For the export of the enzyme through the cell envelope, the intracellular complex with inhibitor IFN must be dissociated | Streptococcus pyogenes serotype M3 |