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Literature summary for 3.2.2.5 extracted from

  • Ghosh, J.; Anderson, P.J.; Chandrasekaran, S.; Caparon, M.G.
    Characterization of Streptococcus pyogenes beta-NAD+ glycohydrolase: re-evaluation of enzymatic properties associated with pathogenesis (2010), J. Biol. Chem., 285, 5683-5694.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.5

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged wild-type and E391Q mutant SPN in Escherichia coli as stable and soluble monomer in the periplasmic space Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
E391Q a catalytically nearly inactive mutant, upon transformation, SPN is toxic to Saccharomyces cerevisiae, whereas a glycohydrolase-inactive SPN allows for viability Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
ADP-ribose product inhibition Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, and kinetic analysis Streptococcus pyogenes
0.188
-
 NAD+ pH 7.4, 37°C, recombinant wild-type enzyme Streptococcus pyogenes
0.294
-
 NAD+ pH 7.4, 37°C, recombinant mutant enzyme Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pyogenes SPN is specific for beta-NAD+ glycohydrolase activity, and does not show ADP-ribosyl cyclase and ADP-ribosyltransferase activities, overview. SPN is unable to catalyze cyclic ADPR hydrolysis, and cannot catalyze methanolysis or transglycosidation ?
-
 ?
NAD+ + H2O Streptococcus pyogenes
-
 ADP-ribose + nicotinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and E391Q mutant SPN from Escherichia coli Streptococcus pyogenes

Reaction

Reaction Comment Organism Reaction ID
NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ SPN shows an ordered uni-bi mechanism, with ADP-ribose being released as a second product. The catalytic mechanism requires an essential glutamic acid, Glu344, to stabilize an oxycarbenium ion intermediate, which subsequently is attacked by a nucleophile, a water molecule Streptococcus pyogenes
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information the Gram-positive pathogen Streptococcus pyogenes injects a beta-NAD+-glycohydrolase into the cytosol of an infected host cell using the cytolysin-mediated translocation pathway Streptococcus pyogenes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SPN is specific for beta-NAD+ glycohydrolase activity, and does not show ADP-ribosyl cyclase and ADP-ribosyltransferase activities, overview. SPN is unable to catalyze cyclic ADPR hydrolysis, and cannot catalyze methanolysis or transglycosidation Streptococcus pyogenes ?
-
 ?
NAD+ + H2O
-
 Streptococcus pyogenes ADP-ribose + nicotinamide
-
 ?

Synonyms

Synonyms Comment Organism
beta-NAD+ glycohydrolase
-
 Streptococcus pyogenes
SPN
-
 Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
 NAD+ pH 7.4, 37°C, recombinant mutant enzyme Streptococcus pyogenes
8390
-
 NAD+ pH 7.4, 37°C, recombinant wild-type enzyme Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Streptococcus pyogenes

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6
-
 ADP-ribose pH 7.4, 37°C, recombinant wild-type enzyme Streptococcus pyogenes

General Information

General Information Comment Organism
additional information the Gram-positive pathogen Streptococcus pyogenes injects a beta-NAD+-glycohydrolase into the cytosol of an infected host cell using the cytolysin-mediated translocation pathway, where SPN accelerates the death of the host cell Streptococcus pyogenes