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Literature summary for 3.2.2.5 extracted from

  • Hara-Yokoyama, M.; Nagatsuka, Y.; Katsumata, O.; Irie, F.; Kontani, K.; Hoshino, S.; Katada, T.; Ono, Y.; Fujita-Yoshigaki, J.; Sugiya, H.; Furuyama, S.; Hirabayashi, Y.
    Complex gangliosides as cell surface inhibitors for the ecto-NAD+ glycohydrolase of CD38 (2001), Biochemistry, 40, 888-895.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in THP-1 cells, selection of transfected cells by G418 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
b-series gangliosides
-
Homo sapiens
GD1a exogenous ganglioside, slight inhibition, inhibition in vivo Homo sapiens
GD1b exogenous ganglioside, inhibition in vivo Homo sapiens
GD3 exogenous ganglioside, inhibition in vivo Homo sapiens
GM1b exogenous ganglioside, slight inhibition, inhibition in vivo Homo sapiens
GT1b exogenous ganglioside, inhibition in vivo, needs to be incorporated into the cell to inhibit CD38, preferably cis interaction, i.e. CD38 and GT1b are located on the same cell, the tandem sialic acid residues linked to the internal galactose of the gangliotetraose core are crucial to the inhibition Homo sapiens
additional information structure of the inhibiting gangliosides, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane protein with a catalytically active extracellular domain Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + H2O Homo sapiens
-
ADP-ribose + nicotinamide
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
the extracellular domain of the leukocyte single transmembrane cell surface antigen CD38 shows NADase activity
-

Reaction

Reaction Comment Organism Reaction ID
NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ the enzyme can also hydrolyse NADP+ to yield phospho-ADP-ribose and nicotinamide, but more slowly Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HL-60 cell leukemic cells, retinoic acid-treated Homo sapiens
-
leukocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + H2O
-
Homo sapiens ADP-ribose + nicotinamide
-
?

Synonyms

Synonyms Comment Organism
CD38
-
Homo sapiens
NADase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens