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Literature summary for 3.2.2.31 extracted from

  • Chang, P.; Madabushi, A.; Lu, A.
    Insights into the role of Val45 and Gln182 of Escherichia coli MutY in DNA substrate binding and specificity (2009), BMC Biochem., 10, 19 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli PR70(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
Q182L the mutant has significantly lower binding and glycosylase activities for adenine:guanine and adenine:7,8-dihydro-8-oxo-guanine mismatches than the wild type enzyme. The mutant has substantially increased affinity towards thymine:guanine, however, it does not exhibit any thymine/guanine or thymine/7,8-dihydro-8-oxo-guanine glycosylase activity Escherichia coli
V45A the mutant has significantly lower binding and glycosylase activities for adenine:guanine and adenine:7,8-dihydro-8-oxo-guanine mismatches than the wild type enzyme. The mutant has substantially increased affinity towards thymine:guanine, however, it does not exhibit any thymine/guanine or thymine/7,8-dihydro-8-oxo-guanine glycosylase activity Escherichia coli
V45A/Q182L the mutant has similar binding affinities to thymine/guanine as the wild type enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phosphocellulose column chromatography, hydroxylapatite column chromatography, heparin column chromatography, and Hitrap-S column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:thymine mismatch + H2O low activity Escherichia coli ?
-
 ?
DNA containing a guanine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a thymine:guanine mismatch + H2O low activity Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
mutY
-
 Escherichia coli