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Literature summary for 3.2.2.31 extracted from

  • Livingston, A.L.; Kundu, S.; Henderson Pozzi, M.; Anderson, D.W.; David, S.S.
    Insight into the roles of tyrosine 82 and glycine 253 in the Escherichia coli adenine glycosylase MutY (2005), Biochemistry, 44, 14179-14190 .
    View publication on PubMed
Search for more literature for 3.2.2.31

Protein Variants

Protein Variants Comment Organism
G253A when 2'-deoxy-2'-fluoroadenosine in duplex paired with guanine or 7,8-dihydro-8-oxo-2'-deoxyguanosine is used, the mutant shows reduced binding affinity. Additionally, compromised glycosylase activity of the mutant enzyme is observed using the nonoptimal guanine:adenine substrate, or at low reaction temperatures Escherichia coli
G253D the mutant has a reduced adenine glycosylase activity and affinity for substrate analogues compared to the wild type enzyme Escherichia coli
Y82C the mutant has a reduced adenine glycosylase activity and affinity for substrate analogues compared to the wild type enzyme. Additionally, compromised glycosylase activity of the mutant enzyme is observed using the nonoptimal guanine:adenine substrate, or at low reaction temperatures Escherichia coli
Y82F when 2’-deoxy-2’-fluoroadenosine in duplex paired with guanine or 7,8-dihydro-8-oxo-2'-deoxyguanosine is used, the mutant shows reduced binding affinity. Additionally, compromised glycosylase activity of the mutant enzyme is observed using the nonoptimal guanine:adenine substrate, or at low reaction temperatures Escherichia coli
Y82L the mutant is unable to discriminate between guanine and 7,8-dihydro-8-oxo-2'-deoxyguanosine when paired with 2'-deoxy-2'-fluoroadenosine Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mispair + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and HiTrap heparin column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mispair + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a guanine:adenine mispair + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
adenine glycosylase
-
 Escherichia coli
mutY
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function the enzyme plays an important role in preventing 7,8-dihydro-8-oxo-2'-deoxyguanosine-associated mutations by removing adenines misincorporated opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine lesions during DNA replication Escherichia coli