Literature summary for 3.2.2.31 extracted from

Lu, A.L.; Lee, C.Y.; Li, L.; Li, X.
Physical and functional interactions between Escherichia coli MutY and endonuclease VIII (2006), Biochem. J., 393, 381-387 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000224	-	DNA containing a 5-hydroxyuracil/adenine mismatch	at pH 7.6 and 37°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA containing a 5-hydroxyuracil/adenine mismatch + H2O	the enzyme has a moderate affinity for DNA containing a 5-hydroxyuracil/adenine mismatch	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
mutY	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000025	-	DNA containing a 5-hydroxyuracil/adenine mismatch	at pH 7.6 and 37°C	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	the enzyme can remove misincorporated adenine opposite guanine or 7,8-dihydro-8-oxoguanine on the template strand during DNA replication. The enzyme has a role in preventing the mutagenic effects of 5-hydroxyuracil	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.07	-	DNA containing a 5-hydroxyuracil/adenine mismatch	at pH 7.6 and 37°C	Escherichia coli

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Release 2023.1 (January 2023)