Literature summary for 3.2.2.30 extracted from

Kim, R.Q.; Offen, W.A.; Davies, G.J.; Stubbs, K.A.
Structural enzymology of Helicobacter pylori methylthioadenosine nucleosidase in the futalosine pathway (2014), Acta Crystallogr. Sect. D, 70, 177-185.

Search for more literature for 3.2.2.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, mutants E14Q (0.1 M HEPES pH 7.5, 25% (w/v) PEG 3350, 0.2 M NaCl), D199N (0.1 M bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M MgCl2-6H2O) and D199A (0.1 M bis-tris pH 6.5, 25% (w/v) PEG 3350, 0.2 M Li2SO4-H2O)	Helicobacter pylori
wild type and mutant enzymes E14Q, D199N, and D199A, sitting drop vapor diffusion method, using 4.0 M NaH2PO4/K2HPO4 pH 7.0 (wild type), 0.1 M HEPES pH 7.5, 25% PEG 3350, 0.2 M NaCl (E14Q), 0.1 M bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M MgCl2-6H2O (D199N), and 0.1 M bis-Tris pH 6.5, 25% (w/v) PEG 3350, 0.2 M Li2SO4-H2O (D199A)	Helicobacter pylori

Protein Variants

Protein Variants	Comment	Organism
D199A	inactive	Helicobacter pylori
D199A	active-site mutant	Helicobacter pylori
D199N	active-site mutant	Helicobacter pylori
D199N	the mutant shows residual activity	Helicobacter pylori
E14Q	active-site mutant	Helicobacter pylori
E14Q	the mutant shows residual activity	Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-amino-6-deoxyfutalosine + H2O	Helicobacter pylori	-	dehypoxanthine futalosine + adenine	-	?
6-amino-6-deoxyfutalosine + H2O	Helicobacter pylori	-	adenine + dehypoxanthinyl futalosine	-	?

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	O24915	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Helicobacter pylori
HisTrap column chromatography and Superdex 75 gel filtration	Helicobacter pylori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-amino-6-deoxyfutalosine + H2O	-	Helicobacter pylori	dehypoxanthine futalosine + adenine	-	?
6-amino-6-deoxyfutalosine + H2O	-	Helicobacter pylori	adenine + dehypoxanthinyl futalosine	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Helicobacter pylori

Synonyms

Synonyms	Comment	Organism
methylthioadenosine nucleosidase	-	Helicobacter pylori
MqnB	-	Helicobacter pylori
MTAN	-	Helicobacter pylori

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Release 2023.1 (January 2023)