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Literature summary for 3.2.2.30 extracted from

  • Siu, K.K.; Lee, J.E.; Sufrin, J.R.; Moffatt, B.A.; McMillan, M.; Cornell, K.A.; Isom, C.; Howell, P.L.
    Molecular determinants of substrate specificity in plant 5-methylthioadenosine nucleosidases (2008), J. Mol. Biol., 378, 112-128.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Codon+ cells Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27462
-
 x * 27462, isoform MATN2, calculated from amino acid sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.38
-
 using adenosine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana
0.83
-
 using 5'-chloroadenosine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana
1.06
-
 using 5'-iodoadenosine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana
1.5
-
 using 5'-butylthioadenosine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana
1.98
-
 using 5'-isobutylthioadenosine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana
2.44
-
 using 5'-phenylthioadenosine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana
6.25
-
 using 5'-methylthioadenosine as substrate, at pH 6.0 and 22°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-butylthioadenosine + H2O
-
 Arabidopsis thaliana ?
-
 ?
5'-chloroadenosine + H2O
-
 Arabidopsis thaliana ?
-
 ?
5'-iodoadenosine + H2O
-
 Arabidopsis thaliana ?
-
 ?
5'-isobutylthioadenosine + H2O
-
 Arabidopsis thaliana ?
-
 ?
5'-methylthioadenosine + H2O
-
 Arabidopsis thaliana ?
-
 ?
5'-phenylthioadenosine + H2O
-
 Arabidopsis thaliana ?
-
 ?
adenosine + H2O
-
 Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27462, isoform MATN2, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2
-
 Arabidopsis thaliana
MTAN2 isoform Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Arabidopsis thaliana