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Literature summary for 3.2.2.29 extracted from

  • Coey, C.T.; Malik, S.S.; Pidugu, L.S.; Varney, K.M.; Pozharski, E.; Drohat, A.C.
    Structural basis of damage recognition by thymine DNA glycosylase Key roles for N-terminal residues (2016), Nucleic Acids Res., 44, 10248-10258 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme construct TDG82-308, sitting drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 6.0 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N140A the mutation completely depletes guanine-thymine glycosylase activity and causes a huge (27000fold) loss in guanine-uracil activity Homo sapiens
T197A the mutation causes a 32fold reduction in guanine-thymine glycosylase activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-carboxylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
5-formylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
thymine-mismatched double-stranded DNA + H2O Homo sapiens
-
 thymine + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O Homo sapiens
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
-
-

Purification (Commentary)

Purification (Comment) Organism
SP Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-carboxylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
5-formylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
thymine-mismatched double-stranded DNA + H2O
-
 Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
TDG
-
 Homo sapiens
thymine DNA glycosylase
-
 Homo sapiens