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Literature summary for 3.2.2.29 extracted from

  • Malik, S.S.; Coey, C.T.; Varney, K.M.; Pozharski, E.; Drohat, A.C.
    Thymine DNA glycosylase exhibits negligible affinity for nucleobases that it removes from DNA (2015), Nucleic Acids Res., 43, 9541-9552 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and in complex with DNA, sitting drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 6.0 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-carboxylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
5-formylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
thymine-mismatched double-stranded DNA + H2O Homo sapiens
-
 thymine + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-carboxylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
5-formylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
additional information the enzyme has negligible affinity for isolated nucleobases Homo sapiens ?
-
 ?
additional information the enzyme removes thymine from mutagenic G-T mispairs caused by 5-methylcytosine deamination and other lesions including uracil and 5-hydroxymethyluracil. In DNA demethylation, the enzyme excises 5-formylcytosine and 5-carboxylcytosine Homo sapiens ?
-
 ?
thymine-mismatched double-stranded DNA + H2O
-
 Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
TDG
-
 Homo sapiens
thymine DNA glycosylase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Homo sapiens