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Literature summary for 3.2.2.29 extracted from

  • Saparbaev, M.; Laval, J.
    3,N4-ethenocytosine, a highly mutagenic adduct, is a primary substrate for Escherichia coli double-stranded uracil-DNA glycosylase and human mismatch-specific thymine-DNA glycosylase (1998), Proc. Natl. Acad. Sci. USA, 95, 8508-8513.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000012
-
uracil-mismatched double-stranded DNA excision of uracil from U/G mismatches Homo sapiens
0.0000128
-
thymine-mismatched double-stranded DNA excision of thymine from T/G mismatches Homo sapiens
0.0000243
-
3,N4-ethenocytosine-mismatched double-stranded DNA excision of 3,N4-ethenocytosine from 3,N4-ethenocytidine/G mismatches Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O Homo sapiens 3,N4-ethenocytosine is recognized and efficiently excised by hTDG. The enzyme may be responsible for the repair of this mutagenic lesion in vivo and be important contributors to genetic stability 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O 3,N4-ethenocytosine is recognized and efficiently excised by hTDG. The enzyme may be responsible for the repair of this mutagenic lesion in vivo and be important contributors to genetic stability Homo sapiens 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
?
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O 3,N4-ethenocytosine is recognized and efficiently excised from the 3,N4-ethenocytosine/G duplex oligonucleotide, when this residue is situated opposite to G. 26.5% of the activity measured with 3,N4-ethenocytosine mismatches is observed with 3,N4-ethenocytosine/A mismatches, 71% with 3,N4-ethenocytosine/A mismatches Homo sapiens 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
?
thymine-mismatched double-stranded DNA + H2O excision of thymine from T/G mismatches Homo sapiens thymine + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O excision of uracil from U/G mismatches Homo sapiens uracil + double-stranded DNA with abasic site
-
?

Synonyms

Synonyms Comment Organism
hTDG
-
Homo sapiens
mismatch-specific thymine-DNA glycosylase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000015
-
thymine-mismatched double-stranded DNA excision of thymine from T/G mismatches Homo sapiens
0.000153
-
3,N4-ethenocytosine-mismatched double-stranded DNA excision of 3,N4-ethenocytosine from 3,N4-ethenocytidine/G mismatches Homo sapiens
0.00035
-
uracil-mismatched double-stranded DNA excision of uracil from U/G mismatches Homo sapiens