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Literature summary for 3.2.2.29 extracted from
- The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells (1993), J. Biol. Chem., 268, 21218-21224.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymine-mismatched double-stranded DNA + H2O | Homo sapiens | the enzyme initiates the repair process by excising the mispaired thymine from the heteroduplex to generate an apyrimidinic site | thymine + double-stranded DNA with abasic site | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q13569 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymine-mismatched double-stranded DNA + H2O | the enzyme initiates the repair process by excising the mispaired thymine from the heteroduplex to generate an apyrimidinic site | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | the enzyme is capable for hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to G/T, the enzyme can remove thymine also from C/T and T/T mispairs in the order of decreasing efficiency: G/T, C/T, T/T. It has no detectable endonucleolytic activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mismatch-specific thymine-DNA N-glycosylase | - |
Homo sapiens |
| thymine-DNA glycosylase | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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