Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13569 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
5-bromocytosine-mismatched double-stranded DNA + H2O | hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site | Homo sapiens | 5-bromouracil + double-stranded DNA with abasic site | - |
? | |
5-chlorouracil-mismatched double-stranded DNA + H2O | hTDG removes 5-chlorouracil 572fold faster than thymine | Homo sapiens | 5-chlorouracil + double-stranded DNA with abasic site | - |
? | |
5-fluorocytosine-mismatched double-stranded DNA + H2O | hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site | Homo sapiens | 5-fluorocytosine + double-stranded DNA with abasic site | - |
? | |
5-fluorouracil-mismatched double-stranded DNA + H2O | hTDG removes 5-fluorouracil 78fold faster than uracil | Homo sapiens | 5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
5-hydroxcytosine-mismatched double-stranded DNA + H2O | hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site | Homo sapiens | 5-hydroxycytosine + double-stranded DNA with abasic site | - |
? | |
thymine-mismatched double-stranded DNA + H2O | cleaves thymine from mutagenic G/T mispairs. Recognizes many additional lesions, and has a strong preference for nucleobases paired with guanine rather than adenine. hTDG avoids cytosine, despite the million-fold excess of normal G/C pairs over G/T mispairs | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hTDG | - |
Homo sapiens |
thymine DNA glycosylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
thymine-mismatched double-stranded DNA | cleavage of thymine from G/T mismatch | Homo sapiens | |
0.043 | - |
uracil-mismatched double-stranded DNA | cleavage of uracil from G/U mismatch | Homo sapiens | |
2.1 | - |
5-chlorouracil-mismatched double-stranded DNA | cleavage of 5-chlorouracil from G/5-chlorouracil mismatch | Homo sapiens | |
3.7 | - |
5-fluorouracil-mismatched double-stranded DNA | cleavage of 5-fluorouracil from G/5-fluorouracil mismatch | Homo sapiens |