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Literature summary for 3.2.2.29 extracted from

  • Bennett, M.T.; Rodgers, M.T.; Hebert, A.S.; Ruslander, L.E.; Eisele, L.; Drohat, A.C.
    Specificity of human thymine DNA glycosylase depends on N-glycosidic bond stability (2006), J. Am. Chem. Soc., 128, 12510-12519.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
-
Homo sapiens 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
?
5-bromocytosine-mismatched double-stranded DNA + H2O hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site Homo sapiens 5-bromouracil + double-stranded DNA with abasic site
-
?
5-chlorouracil-mismatched double-stranded DNA + H2O hTDG removes 5-chlorouracil 572fold faster than thymine Homo sapiens 5-chlorouracil + double-stranded DNA with abasic site
-
?
5-fluorocytosine-mismatched double-stranded DNA + H2O hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site Homo sapiens 5-fluorocytosine + double-stranded DNA with abasic site
-
?
5-fluorouracil-mismatched double-stranded DNA + H2O hTDG removes 5-fluorouracil 78fold faster than uracil Homo sapiens 5-fluorouracil + double-stranded DNA with abasic site
-
?
5-hydroxcytosine-mismatched double-stranded DNA + H2O hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site Homo sapiens 5-hydroxycytosine + double-stranded DNA with abasic site
-
?
thymine-mismatched double-stranded DNA + H2O cleaves thymine from mutagenic G/T mispairs. Recognizes many additional lesions, and has a strong preference for nucleobases paired with guanine rather than adenine. hTDG avoids cytosine, despite the million-fold excess of normal G/C pairs over G/T mispairs Homo sapiens thymine + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O
-
Homo sapiens uracil + double-stranded DNA with abasic site
-
?

Synonyms

Synonyms Comment Organism
hTDG
-
Homo sapiens
thymine DNA glycosylase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0036
-
thymine-mismatched double-stranded DNA cleavage of thymine from G/T mismatch Homo sapiens
0.043
-
uracil-mismatched double-stranded DNA cleavage of uracil from G/U mismatch Homo sapiens
2.1
-
5-chlorouracil-mismatched double-stranded DNA cleavage of 5-chlorouracil from G/5-chlorouracil mismatch Homo sapiens
3.7
-
5-fluorouracil-mismatched double-stranded DNA cleavage of 5-fluorouracil from G/5-fluorouracil mismatch Homo sapiens