Literature summary for 3.2.2.29 extracted from

Sibghat-Ullah; Gallinari, P.; Xu, Y.Z.; Goodman, M.F.; Bloom, L.B.; Jiricny, J.; Day, R.S. 3rd.
Base analog and neighboring base effects on substrate specificity of recombinant human G:T mismatch-specific thymine DNA-glycosylase (1996), Biochemistry, 35, 12926-12932.

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Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13569	recombinant	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thymine-mismatched double-stranded DNA + H2O	45-bp DNA heteroduplexes that bear single G/T, O6-methyguanine, 2,6-diaminopurine/T, 2-amino-6-(methylamino)-purine/T, 2-aminopurine/T, and G/O4-methylthymine mispairs. The bases 5' to the poorly matched G are altered in selected G/T substrates to yield mispairs in four different contexts, ApG, CpG, GpG, and TpG. The recombinant thymine glycosylase is incubated with the 45-bp DNA substrates, each labeled at the 5'-terminus of the strand containing the mismatched T. The rate of incision is greatest with DNA containing the G/T mispair followed by the DNA containing the O6-methylguanine/T mispair and the DNA with the 2-amino-6-(methylamino)purine/T mispair. The extent of reaction is 90%, 40%, and 20% respectively. DNA substrates containing 2,6-diaminopurine/T, 2-aminopurine/T, and G/O4-methylthymine mispairs are not incised. The amount of incision of the 45-bp DNA substrates containing G/T mispairs in the CpG context is 3-12fold greater than in the TpG, GpG, and ApG contexts	Homo sapiens	thymine + double-stranded DNA with abasic site	-	?

Synonyms

Synonyms	Comment	Organism
G:T mismatch-specific thymine DNA-glycosylase	-	Homo sapiens

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Release 2023.1 (January 2023)