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Literature summary for 3.2.2.24 extracted from

  • Nielsen, G.M.; Bao, Y.; Roberts, G.P.; Ludden, P.W.
    Purification and characterization of an oxygen-stable form of dinitrogenase reductase-activating glycohydrolase from Rhodospirillum rubrum (1994), Biochem. J., 302, 801-806.
    View publication on PubMedView publication on EuropePMC
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General Stability

General Stability Organism
Mn2+ renders the enzyme more susceptible to inactivation by dithionite in air, not Ca2+, Fe2+ or Mg2+, ADPribose protects, not ADP Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
ADPribose
-
 Rhodospirillum rubrum
Ca2+
-
 Rhodospirillum rubrum
Dithionite in air, inactivation Rhodospirillum rubrum
H2O2 inactivation, to the same extent as dithionite in air Rhodospirillum rubrum
additional information no inhibition by ADP Rhodospirillum rubrum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no covalently bound inorganic cofactors, inductively coupled plasma emission analysis, detection limit for Mn2+: 0.00005 mM Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex Rhodospirillum rubrum
-
 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Oxidation Stability

Oxidation Stability Organism
attempts to purify the enzyme using aerobic buffer are partly successful, aerobic DEAE-cellulose chromatography results in rapid loss of activity, 30 min stable to exposure to air in buffer containing DTT in the absence of dithionite, in its presence decrease of activity with time, bovine serum albumin does not protect Rhodospirillum rubrum

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.1
-
 strain UR1, anaerobic preparation, prepared in the presence of 1 mM dithionite, pH 7.5 Rhodospirillum rubrum
56.6
-
 strain UR276, anaerobic preparation, prepared in the presence of 1 mM dithionite, pH 7.5 Rhodospirillum rubrum
138
-
 strain UR276, anaerobic preparation, pH 7.5 Rhodospirillum rubrum

Storage Stability

Storage Stability Organism
4°C, purified preparation exposed to air in the absence of dithionite, 22 h Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex
-
 Rhodospirillum rubrum ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?