Protein Variants | Comment | Organism |
---|---|---|
N168D | 0.2% of wild-type activity | Escherichia coli |
N168Q | 9.5% of wild-type activity | Escherichia coli |
R108K | 1.9% of wild-type activity | Escherichia coli |
R108L | 0.3% of wild-type activity | Escherichia coli |
R108Q | 0.3% of wild-type activity | Escherichia coli |
R258A | 0.4% of wild-type activity | Escherichia coli |
R258K | 0.3% of wild-type activity | Escherichia coli |
R258Q | 6.2% of wild-type activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000063 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | mutant R258Q, pH 7.5, 30°C | Escherichia coli | |
0.0000081 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | wild-type, pH 7.5, 30°C | Escherichia coli | |
0.0000081 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | mutant N168Q, pH 7.5, 30°C | Escherichia coli | |
0.000018 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | mutant R108K, pH 7.5, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05523 | - |
- |
Geobacillus stearothermophilus | P84131 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
23mer oligonucleotide duplex containing 8-oxoguanine + H2O | - |
Escherichia coli | 8-oxoguanine + oligonucleotide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | mutant R108K, pH 7.5, 30°C | Escherichia coli | |
0.08 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | mutant R258Q, pH 7.5, 30°C | Escherichia coli | |
0.16 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | mutant N168Q, pH 7.5, 30°C | Escherichia coli | |
1.7 | - |
23mer oligonucleotide duplex containing 8-oxoguanine | wild-type, pH 7.5, 30°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | Fpg selectively facilitates eversion of 8-oxoguanine by stabilizing several intermediate states, helping the rapidly sliding enzyme avoid full extrusion of every encountered base for interrogation | Escherichia coli |
physiological function | Fpg selectively facilitates eversion of 8-oxoguanine by stabilizing several intermediate states, helping the rapidly sliding enzyme avoid full extrusion of every encountered base for interrogation | Geobacillus stearothermophilus |