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Literature summary for 3.2.2.23 extracted from

  • Duwat, P.; de Oliveira, R.; Ehrlich, S.D.; Boiteux, S.
    Repair of oxidative DNA damage in gram-positive bacteria: the Lactococcus lactis Fpg protein (1995), Microbiology, 141, 411-417.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
fpg-L gene is cloned, sequenced and overexpressed in Escherichia coli BH410, expression in Escherichia coli fpg and mutY mutants suppresses their spontaneous GC-TA mutator phenotype Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains one zinc per enzyme molecule Lactococcus lactis
Zn2+ contains a single zinc finger motif near the C-terminus Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
active form Lactococcus lactis
31300
-
1 * 31300, calculated from the amino acid sequence Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Lactococcus lactis important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species ?
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis P42371 subspecies cremoris ML3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, overexpressed in Escherichia coli BH410 Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Lactococcus lactis ?
-
?
DNA + H2O important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species Lactococcus lactis ?
-
?
DNA containing 8-hydroxyguanine residues + H2O 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue Lactococcus lactis 8-hydroxyguanine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites Lactococcus lactis 8-hydroxyguanine + DNA
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
-
Lactococcus lactis 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Lactococcus lactis
monomer 1 * 31300, calculated from the amino acid sequence Lactococcus lactis

Synonyms

Synonyms Comment Organism
Fpg-L
-
Lactococcus lactis

pI Value

Organism Comment pI Value Maximum pI Value
Lactococcus lactis
-
-
9