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Literature summary for 3.2.2.23 extracted from

  • Chetsanga, C.J.; Lozon, M.; Makaroff, C.; Savage, L.
    Purification and characterization of Escherichia coli formamidopyrimidine-DNA glycosylase that excises damaged 7-methylguanine from deoxyribonucleic acid (1981), Biochemistry, 20, 5201-5207.
    View publication on PubMed

General Stability

General Stability Organism
50% glycerol destabilizes during storage, concentration of above 10% cause a 60% reduction in activity Escherichia coli
complete loss of activity if the enzyme stored at -20°C or -80°C is subsequently thawed for assaying Escherichia coli
rapid loss of activity when a conventional pressure cell is used to concentrate enzyme Escherichia coli
unstable in low ionic strength solutions Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-Nitroso-2,4,6-triaminopyrimidine product inhibition Escherichia coli
Formamidopyrimidine product inhibition Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
KCl stimulates Escherichia coli
KCl optimum concentration: 0.1 M Escherichia coli
KH2PO4 pH 7.4, stimulates, optimum concentration: 0.05 M Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
8000fold Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
0°C, buffered 0.5 M KCl, 1 week, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing ring-opened N7-methylguanine residues + H2O double-stranded DNA is preferred to single-stranded DNA Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
?
additional information no cleavage of 3-methyladenine, uracil, intact 7-methylguanine from DNA Escherichia coli ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
purified enzyme is very sensitive to extreme temperature fluctuations Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 8
-
Escherichia coli
7.4
-
assay at Escherichia coli