General Stability | Organism |
---|---|
50% glycerol destabilizes during storage, concentration of above 10% cause a 60% reduction in activity | Escherichia coli |
complete loss of activity if the enzyme stored at -20°C or -80°C is subsequently thawed for assaying | Escherichia coli |
rapid loss of activity when a conventional pressure cell is used to concentrate enzyme | Escherichia coli |
unstable in low ionic strength solutions | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Nitroso-2,4,6-triaminopyrimidine | product inhibition | Escherichia coli | |
Formamidopyrimidine | product inhibition | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulates | Escherichia coli | |
KCl | optimum concentration: 0.1 M | Escherichia coli | |
KH2PO4 | pH 7.4, stimulates, optimum concentration: 0.05 M | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
8000fold | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, buffered 0.5 M KCl, 1 week, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing ring-opened N7-methylguanine residues + H2O | double-stranded DNA is preferred to single-stranded DNA | Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? | |
additional information | no cleavage of 3-methyladenine, uracil, intact 7-methylguanine from DNA | Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme is very sensitive to extreme temperature fluctuations | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8 | - |
Escherichia coli |
7.4 | - |
assay at | Escherichia coli |