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Literature summary for 3.2.2.23 extracted from

  • O'Connor, T.R.; Laval, J.
    Physical association of the 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites (1989), Proc. Natl. Acad. Sci. USA, 86, 5222-5226.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.23

Cloned(Commentary)

Cloned (Comment) Organism
fpg gene Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 hosting pFPG60 plasmid
-

Purification (Commentary)

Purification (Comment) Organism
copurified with apurinic/apyrimidinic-nicking activity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0423
-
 pH 7.6, 37°C, Fapy-DNA glycosylase activity Escherichia coli
0.5
-
 pH 7.8, 37°C, apurinic/apyrimidinic-nicking activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O excises purine bases with ring-opened imidazoles, associated activity that nicks DNA at apurinic/apyrimidinic sites, mechanism of cleavage involves beta elimination Escherichia coli ?
-
 ?
DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
 ?
DNA containing ring-opened N7-methylguanine residues + H2O
-
 Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Escherichia coli