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Literature summary for 3.2.2.22 extracted from

  • Sturm, M.B.; Schramm, V.L.
    Detecting ricin: sensitive luminescent assay for ricin A-chain ribosome depurination kinetics (2009), Anal. Chem., 81, 2847-2853.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.22

Application

Application Comment Organism
analysis facile analysis of RIP catalytic activity will have applications in plant toxin detection, inhibitor screens, mechanistic analysis of depurinating agents on oligonucleotides and intact ribosomes, and in cancer immunochemotherapy Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Ricinus communis
0.00006
-
 yeast 60S rRNA pH 4.0 Ricinus communis
0.0001
-
 rabbit reticulocyte 80S rRNA pH 4.0 Ricinus communis
0.0022
-
 stem-loop substrate A-10 pH 4.0 Ricinus communis
0.0103
-
 A-14 2-dA stem-loop RNA/DNA hybrid pH 4.0 Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Ricinus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
A-14 2-dA stem-loop RNA/DNA hybrid + H2O
-
 Ricinus communis ?
-
 ?
additional information ricin toxin A-chain catalyzes the hydrolytic depurination of a single base from a GAGA tetraloop of eukaryotic ribosomal RNA to release a single adenine from the sarcin-ricin loop. It catalyzes depurination of 80S rabbit reticulocyte ribosomes and 60S yeast ribosomes by RTA, substrates are stem-loop substrate A-10 and A-14 2-dA stem-loop RNA/DNA hybrid, in general 6mer to 18mer stem-loop DNA and RNA 28S SRL mimic oligonucleotides. Development of a high-sensitive quantitative adenine detection method for enzyme activity measurement through coupling of RIP activity with adenine phosphoribosyl transferase, EC 2.4.2.7, and pyruvate orthophosphate dikinase, EC 2.7.9.1, in a colimetric assay, discontinous and continouos formats, optimization, overview. Deoxyadenosine at the depurination site, GdAGA, of RNA stem-loop oligonucleotides increases the catalytic efficiency by RTA a factor of about 4 Ricinus communis ?
-
 ?
rabbit reticulocyte 80S rRNA + H2O
-
 Ricinus communis ?
-
 ?
stem-loop substrate A-10 + H2O
-
 Ricinus communis ?
-
 ?
yeast 60S rRNA + H2O
-
 Ricinus communis ?
-
 ?

Synonyms

Synonyms Comment Organism
ribosome-inactivating proteins
-
 Ricinus communis
ricin
-
 Ricinus communis
ricin toxin A-chain
-
 Ricinus communis
RIP
-
 Ricinus communis
RTA
-
 Ricinus communis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.078
-
 stem-loop substrate A-10 pH 4.0 Ricinus communis
18.5
-
 A-14 2-dA stem-loop RNA/DNA hybrid pH 4.0 Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 the pH 4.0 catalytic optimum of RTA on 6mer to 18mer stem-loop DNA and RNA 28S SRL mimic oligonucleotides is known to result from the protonation of two ionizable residues on substrate and/or RTA Ricinus communis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 RTA is inactive at pH 7.0 Ricinus communis

General Information

General Information Comment Organism
malfunction the enzyme causes inhibition of protein synthesis by loss of elongation factor binding resulting in cell death Ricinus communis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information catalysis of 80S ribosome by RTA approaches the diffusion rate limit for enzymatic reactions Ricinus communis