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Literature summary for 3.2.2.22 extracted from

  • Choudhary, N.; Kapoor, H.C.; Lodha, M.L.
    Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea x buttiana (2008), J. Biosci., 33, 91-101.
    View publication on PubMed
Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli strain DH5alpha with pDrive vector for cloning and strain TB1 with expression vector pMAL-c2X for expression Bougainvillea x buttiana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE, recombinant BBAP1 cleaved off the fusion protein Bougainvillea x buttiana
35490
-
 calculated value of 319 amino acids of BBAP1, signal peptide sequence of 36 amino acids of 3.89 kDa at N-terminus Bougainvillea x buttiana
42500
-
 SDS-PAGE, maltose-binding protein as partial degradation product of fusion protein Bougainvillea x buttiana
78000
-
 SDS-PAGE, recombinant fusion protein of BBAP1 and maltose-binding protein Bougainvillea x buttiana

Organism

Organism UniProt Comment Textmining
Bougainvillea x buttiana Q4U473
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity column chromatography with a 10 x 2.5 cm column packed with amylose resin specific for maltose-binding protein, eluted pure protein cleaved and dialyzed against 20 mM sodium acetate, pH 5.2, with 0.01% sodium azide, loaded to Sephadex G-100 column Bougainvillea x buttiana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Bougainvillea x buttiana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 84% inhibition of tobacco mosaic virus on Nicotiana glutinosa leaves, determined by comparing lesions on BBAP1 treated with untreated leaves as control Bougainvillea x buttiana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rRNA + H2O activitiy test with ribosomes isolated from Nicotiana tabacum leaves, antiviral activity tested with Nicotiana glutinosa leaves treated with enzyme and subsequently with tobacco mosaic virus Bougainvillea x buttiana ?
-
 ?

Synonyms

Synonyms Comment Organism
BBAP1
-
 Bougainvillea x buttiana
Bougainvillea xbuttiana antiviral protein 1
-
 Bougainvillea x buttiana