Cloned (Comment) | Organism |
---|---|
Escherichia coli strain DH5alpha with pDrive vector for cloning and strain TB1 with expression vector pMAL-c2X for expression | Bougainvillea x buttiana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE, recombinant BBAP1 cleaved off the fusion protein | Bougainvillea x buttiana |
35490 | - |
calculated value of 319 amino acids of BBAP1, signal peptide sequence of 36 amino acids of 3.89 kDa at N-terminus | Bougainvillea x buttiana |
42500 | - |
SDS-PAGE, maltose-binding protein as partial degradation product of fusion protein | Bougainvillea x buttiana |
78000 | - |
SDS-PAGE, recombinant fusion protein of BBAP1 and maltose-binding protein | Bougainvillea x buttiana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bougainvillea x buttiana | Q4U473 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity column chromatography with a 10 x 2.5 cm column packed with amylose resin specific for maltose-binding protein, eluted pure protein cleaved and dialyzed against 20 mM sodium acetate, pH 5.2, with 0.01% sodium azide, loaded to Sephadex G-100 column | Bougainvillea x buttiana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Bougainvillea x buttiana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
84% inhibition of tobacco mosaic virus on Nicotiana glutinosa leaves, determined by comparing lesions on BBAP1 treated with untreated leaves as control | Bougainvillea x buttiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rRNA + H2O | activitiy test with ribosomes isolated from Nicotiana tabacum leaves, antiviral activity tested with Nicotiana glutinosa leaves treated with enzyme and subsequently with tobacco mosaic virus | Bougainvillea x buttiana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BBAP1 | - |
Bougainvillea x buttiana |
Bougainvillea xbuttiana antiviral protein 1 | - |
Bougainvillea x buttiana |