Literature summary for 3.2.2.22 extracted from

Waelchli, S.; Aasheim, H.C.; Skanland, S.S.; Spilsberg, B.; Torgersen, M.L.; Rosendal, K.R.; Sandvig, K.
Characterization of clathrin and Syk interaction upon Shiga toxin binding (2009), Cell. Signal., 21, 1161-1168.

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Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli BL21 and TKB1	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
Y1477F	binding site for tyrosine kinase Syk at clathrin chain	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	transfected Ramos cell cultures	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	phosphorylation of clathrin heavy chain, mediated by tyrosine kinase Syk activity after binding to its cell receptor Gb3	Escherichia coli

Purification (Commentary)

Purification (Comment)	Organism
sonicated, cleared lysate of cell culture in 20 mM Tris buffer, pH 8.0 with 100 mM NaCl, 1 mM Na-vanadate and protease inhibitors (GST-buffer), incubated with glutathione-Sepharose beads, transferred to column, washed with 0.1 M Tris buffer, pH 7.8 with 0.5 M NaCl, with GST-buffer containing 0.35% Triton X-100, and GST-buffer alone	Escherichia coli

Synonyms

Synonyms	Comment	Organism
Shiga toxin	-	Escherichia coli
Stx	-	Escherichia coli

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Release 2023.1 (January 2023)