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Literature summary for 3.2.2.22 extracted from

  • Quinones, B.; Massey, S.; Friedman, M.; Swimley, M.S.; Teter, K.
    Novel cell-based method to detect Shiga toxin 2 from Escherichia coli O157:H7 and inhibitors of toxin activity (2009), Appl. Environ. Microbiol., 75, 1410-1416.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
diagnostics identification of toxin with Vero-2dEGFP assay, threshold for toxin detection 10 picog/ml, and identification of toxin inhibitors, 10% glycerol enhances fluorescence signal Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
grape pomace extract i.e. grape skin extract, dilutions of 0.5 mg/ml extract, 200fold greater EC50 (50% effective concentration) Escherichia coli
grape seed extract dilutions of 0.5 mg/ml extract, 6700fold greater EC50(50% effective concentration) Escherichia coli
additional information red wine concentrate (dilutions of 1 mg/ml) and caffeic acid (dilutions of 0.5 mg/ml) without inhibitory function in the assay Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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O157:H7 containing Shiga toxins type 1 and 2 (Stx1, Stx2), strains RM1697 (Stx1 + Stx2 producing O157 strain), RM4876 (non-Stx-producing o157 strain), RM5034 (non-pathogenic strain)
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
about 90% retained protein synthesis activity in assay cells and about 70% fluorescence by 0.001 ng/ml Stx2, about 50% activity and fluorescence by 0.1 ng/ml Stx2, about 30% activity and fluorescence by 100 ng/ml Stx2, Shiga toxin type 1 and 2 producing cell culture of strain RM1697 has similar dose-response curve as Stx2 Escherichia coli

Synonyms

Synonyms Comment Organism
Shiga toxin
-
Escherichia coli
Shiga toxin type 1
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Escherichia coli
Shiga toxin type 2
-
Escherichia coli
Stx1
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Escherichia coli
Stx2
-
Escherichia coli