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Literature summary for 3.2.2.22 extracted from
- Identifitcation of the RNA N-glycosidase activity of ricin in castor bean extracts by an electrochemiluminescence-based assay (2008), Anal. Biochem., 378, 87-89.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | differentiation of castor bean toxins from other N-glycosidase toxins depending on the signal to background ratio of the substrate GdAAA to GdAGA, castor bean extract can be distinguished from jequirity seed extract (Abrus precatorius) | Ricinus communis |
| diagnostics | differentiation of castor bean toxins from other N-glycosidase toxins depending on the signal to background ratio of the substrate GdAAA to GdAGA, castor bean extract can be distinguished from jequirity seed extract, ricin equivalent activity for jequirity seed 200-fold higher than with immunoassay | Abrus precatorius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Abrus precatorius | removal of specific adenine of ribosomal RNA | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Abrus precatorius | - |
jequirity seed | - |
| Ricinus communis | - |
castor bean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| particle removal by centrifugation of ground material and concentration by dialysis against PEG | Ricinus communis |
| particle removal by centrifugation of ground material and concentration by dialysis against PEG | Abrus precatorius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Abrus precatorius | - |
| seed | bean | Ricinus communis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'/biotin/AGCGGGAGAGdAAAUCUCCC + H2O | RNA GdAAA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal | Ricinus communis | 5'/biotin/AGCGGGAGAG + ? | - |
? | |
| 5'/biotin/AGCGGGAGAGdAAAUCUCCC + H2O | RNA GdAAA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal | Abrus precatorius | 5'/biotin/AGCGGGAGAG + ? | - |
? | |
| 5'/biotin/AGCGGGAGAGdAGAUCUCCC + H2O | RNA GdAGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal | Ricinus communis | 5'/biotin/AGCGGGAGAG + ? | - |
? | |
| 5'/biotin/AGCGGGAGAGdAGAUCUCCC + H2O | RNA GdAGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal | Abrus precatorius | 5'/biotin/AGCGGGAGAG + ? | - |
? | |
| 5'/biotin/AGCGGGAGAGdUGAUCUCCC + H2O | RNA GdUGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal to test for false positive signal | Ricinus communis | 5'/biotin/AGCGGGAGAG + ? | - |
? | |
| 5'/biotin/AGCGGGAGAGdUGAUCUCCC + H2O | RNA GdUGA substrate hybridized with ruthenylated oligodeoxynucleotide 50/Ru/TTTTTdAdCdCTdCTdCTdCdGdCTdC to give a electrochemiluminescence signal to test for false positive signal | Abrus precatorius | 5'/biotin/AGCGGGAGAG + ? | - |
? | |
| additional information | removal of specific adenine of ribosomal RNA | Abrus precatorius | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| abrin | - |
Abrus precatorius |
| ricin | - |
Ricinus communis |
| RNA N-glycosidase | - |
Ricinus communis |
| RNA N-glycosidase | - |
Abrus precatorius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
optimal temperature of ricin activity at 1 ng/ml based on signal-to-background ratios, 1.8fold higher than at 37°C, and 1.2fold higher than at 57°C | Ricinus communis |
| 48 | - |
optimal temperature of ricin activity at 1 ng/ml based on signal-to-background ratios, 1.8fold higher than at 37°C, and 1.2fold higher than at 57°C | Abrus precatorius |
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