Any feedback?
Literature summary for 3.2.2.22 extracted from
- Bacterial expression and enzymatic activity analysis of ME1, a ribosome-inactivating protein from Mirabilis expansa (2005), Protein Expr. Purif., 40, 142-151.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged ME1 in Escherichia coli strain BL21(DE3) for which ME1 is nontoxic | Mirabilis expansa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 28S rRNA + H2O | Mirabilis expansa | inactivation of protein translation in targeted cells, e.g. bacterial cells, where the toxin is transported into the cytosol | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mirabilis expansa | Q8GV51 | andean root crop, type I ribosome-inactivating protein ME1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged ME1 from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography, using a polyclonal anti-ME1 antibody resin, and anion exchange chromatography | Mirabilis expansa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | storage root | Mirabilis expansa | - |
| storage tissue | - |
Mirabilis expansa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 28S rRNA + H2O | inactivation of protein translation in targeted cells, e.g. bacterial cells, where the toxin is transported into the cytosol | Mirabilis expansa | ? | - |
? | |
| 28S rRNA + H2O | Saccharomyces cerevisiae ribosomes, depurination of rRNA | Mirabilis expansa | ? | - |
? | |
| additional information | no activity with Escherichia coli ribosomes by native and recombinant enzyme | Mirabilis expansa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure modeling of ME1 | Mirabilis expansa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ME1 | - |
Mirabilis expansa |
| type I ribosome-inactivating protein | - |
Mirabilis expansa |
| type I RIP | - |
Mirabilis expansa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mirabilis expansa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Mirabilis expansa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
