Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | expression of gene RIP3:2 is up-regulated by drought | Zea mays |
Cloned (Comment) | Organism |
---|---|
gene RIP3:1 is expressed under control of the Opaque-2 promoter | Zea mays |
gene RIP3:2, genomic DNA library screening, DNA and amino acid sequence determination and analyis, RNA mapping and expression profiling, expression is not under control of the Opaque-2 promoter, expression of gene RIP3:2 encoding isozyme RIP2 in Escherichia coli strain BL21(DE3) as N-terminally His-tagged protein | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the isozyme accumulates as inactive precursor protein | Zea mays | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Zea mays | the isozyme RIP1 has defense-related function in maize plants | ? | - |
? | |
additional information | Zea mays | the isozyme RIP2 has defense-related function in maize plants | ? | - |
? | |
rRNA + H2O | Zea mays | inhibition of translation and protein synthesis, cytotoxic | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
strains W64A and W64A o2, gene RIP3:2 encoding the type 3 RIP isozyme RIP2 | - |
Zea mays | P25891 | strains W64A and W64A o2, gene RIP3:1 encoding the type 3 RIP isozyme RIP1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | isozyme RIP1 is synthesized as inactive precursor protein B-32 or proRIP, which is activated by proteolytic cleavage into two polypeptide chains forming the dimeric enzyme, the enzyme can be activated by papain | Zea mays |
proteolytic modification | isozyme RIP2 is synthesized as inactive precursor protein proRIP2, which is activated by proteolytic cleavage into two polypeptide chains forming the dimeric enzyme, the enzyme can be activated by papain | Zea mays |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged isozyme RIP2 from Escherichia coli strain BL21(DE3) | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
kernel | - |
Zea mays | - |
leaf | - |
Zea mays | - |
root | - |
Zea mays | - |
shoot | - |
Zea mays | - |
silk | - |
Zea mays | - |
tassel | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the isozyme RIP1 has defense-related function in maize plants | Zea mays | ? | - |
? | |
additional information | the isozyme RIP2 has defense-related function in maize plants | Zea mays | ? | - |
? | |
additional information | isozyme RIP1 shows relatively low cytotoxic activity and ressembles type I RIPs concerning its substrate specificity | Zea mays | ? | - |
? | |
additional information | isozyme RIP2 shows relatively low cytotoxic activity and ressembles type I RIPs concerning its substrate specificity | Zea mays | ? | - |
? | |
rRNA + H2O | inhibition of translation and protein synthesis, cytotoxic | Zea mays | ? | - |
? | |
rRNA + H2O | depurination of specific sites in rRNA | Zea mays | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | type III RIP isozyme RIP1 forms dimers and ressembles type II RIPs concerning its subunit structure | Zea mays |
dimer | type III RIP isozyme RIP2 forms dimers and ressembles type II RIPs concerning its subunit structure | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
B-32 | - |
Zea mays |
ribosome-inactivating protein 3 | - |
Zea mays |
RIP1 | - |
Zea mays |
RIP2 | - |
Zea mays |
RNA-specific N-glycosidase | - |
Zea mays |
rRNA N-glycosidase | - |
Zea mays |
type III ribosome-inactivating protein | - |
Zea mays |
type III RIP | - |
Zea mays |